土耳其绵羊品种DNA多态性的随机扩增多态性DNA分析
RAPD analysis of DNA polymorphism in Turkish sheep breeds.
作者信息
Elmaci Cengiz, Oner Yasemin, Ozis Seniz, Tuncel Erdogan
机构信息
Animal Science Department, Faculty of Agriculture, Uludag University, 16059, Bursa, Turkey.
出版信息
Biochem Genet. 2007 Oct;45(9-10):691-6. doi: 10.1007/s10528-007-9106-x. Epub 2007 Sep 19.
The genetic diversity and phylogenetic relationships of 108 individual sheep from three Turkish sheep breeds (Kivircik, Gökçeada, and Sakiz) were studied using RAPD analysis. Polymorphisms within and between populations were assayed using 15 random primers, and 82 loci were amplified ranging from 250 to 2,500 bp. The percentage of polymorphic loci was found to be 80.49, 78.05, and 73.17%, for Kivircik, Gökçeada, and Sakiz sheep breeds, respectively. Total genetic diversity was 0.2265, and the average coefficient of genetic differentiation was 0.1181. Genetically, the Gökçeada breed was more closely related to the Sakiz breed than to the Kivircik breed.
利用随机扩增多态性DNA（RAPD）分析研究了来自三个土耳其绵羊品种（基尔维吉克、戈克恰达和萨基兹）的108只个体绵羊的遗传多样性和系统发育关系。使用15条随机引物检测群体内和群体间的多态性，扩增出82个位点，大小在250至2500 bp之间。结果发现，基尔维吉克、戈克恰达和萨基兹绵羊品种的多态位点百分比分别为80.49%、78.05%和73.17%。总遗传多样性为0.2265，平均遗传分化系数为0.1181。从遗传学角度来看，戈克恰达品种与萨基兹品种的关系比与基尔维吉克品种的关系更密切。
Zotero 插件