用高效液相色谱-串联质谱法对千里光中吡咯里西啶生物碱阿多尼弗林碱进行定量分析。

Quantitative analysis by HPLC-MS2 of the pyrrolizidine alkaloid adonifoline in Senecio scandens.

作者信息

Zhang Fang, Wang Chang-Hong, Wang Wan, Chen Lu-Xin, Ma Hong-Yan, Zhang Chao-Feng, Zhang Mian, Bligh S W Annie, Wang Zheng-Tao

机构信息

Key Laboratory of Standardisation of Chinese Medicines of Ministry of Education, Shanghai University of Traditional Chinese Medicine, Zhangjiang Hi-Tech Park, Shanghai 201203, People's Republic of China.

出版信息

Phytochem Anal. 2008 Jan-Feb;19(1):25-31. doi: 10.1002/pca.1009.

DOI:10.1002/pca.1009

PMID:17879229

Abstract

A quantitative method using HPLC-MS(2) has been developed for the determination of adonifoline, one of the retronecine-type hepatotoxic pyrrolizidine alkaloids in Senecio scandens Buch.-Ham. ex D. Don., a traditional Chinese herb. Using an orthogonal design test, a simple and rapid sample extraction method was developed. HPLC analysis was conducted using a C(18) column as stationary phase and a mixture of acetonitrile and aqueous formic acid as mobile phase. Good linearity for adonifoline was found in the concentration range 0.12-4.18 microg/mL, and the HPLC-MS/MS method was shown to be appropriate, in terms of sensitivity, precision and reproducibility. The quantities of adonifoline in extracts of 18 plant samples from different collection sources and from different parts (flowers, leaves, thick stems, slim stems and roots) of S. scandens were determined using the newly developed HPLC/MS(2) analysis.

摘要

已开发出一种使用高效液相色谱-串联质谱法（HPLC-MS(2)）的定量方法，用于测定传统中药千里光（Senecio scandens Buch.-Ham. ex D. Don.）中倒千里光碱型肝毒性吡咯里西啶生物碱之一的阿多尼弗林碱。采用正交设计试验，开发了一种简单快速的样品提取方法。HPLC分析采用C(18)柱作为固定相，乙腈和甲酸水溶液的混合物作为流动相。阿多尼弗林碱在0.12 - 4.18μg/mL浓度范围内具有良好的线性，并且HPLC-MS/MS方法在灵敏度、精密度和重现性方面表现适宜。使用新开发的HPLC/MS(2)分析法测定了来自不同采集源以及千里光不同部位（花、叶、粗茎、细茎和根）的18个植物样品提取物中阿多尼弗林碱的含量。