Rehnberg Johanna, Zendehrokh Nooreldin, Dejmek Annika
Department of Laboratory Medicine, Section of Pathology, Malmö University Hospital, Lund University, Malmö, Sweden.
Anal Quant Cytol Histol. 2007 Aug;29(4):217-20.
To determine the proliferation rates of mesothelial cells in metastatic and benign effusions.
Immunohistochemistry was performed on formalin-fixed pellets from 16 malignant and 9 benign clinical effusions. Dual staining with antibodies against Ki-67 (MIB-1) and desmin was applied to all effusions to differentiate between benign mesothelial cells and malignant cells, and the proportions of desmin+/Ki-67+ and desmin+/Ki-67- cells were calculated.
In 7 malignant effusions no proliferating mesothelial cells were found, whereas some rate of proliferation could always be demonstrated in mesothelial cells in the benign effusions. Further, the median proportions of proliferating cells, malignant 2% vs. benign 11%, differed significantly.
To our knowledge this finding has not been previously described, and it may have implications for both cytologic diagnosis and the understanding of tumor biology and the interaction between tumor cells and mesothelial cells.
确定间皮细胞在转移性积液和良性积液中的增殖率。
对来自16例恶性和9例良性临床积液的福尔马林固定沉淀物进行免疫组织化学检测。对所有积液应用抗Ki-67（MIB-1）和结蛋白抗体进行双重染色，以区分良性间皮细胞和恶性细胞，并计算结蛋白+/Ki-67+和结蛋白+/Ki-67-细胞的比例。
在7例恶性积液中未发现增殖的间皮细胞，而在良性积液的间皮细胞中总能显示出一定的增殖率。此外，增殖细胞的中位数比例，恶性为2%，良性为11%，差异显著。
据我们所知，这一发现此前尚未见报道，它可能对细胞学诊断以及肿瘤生物学的理解以及肿瘤细胞与间皮细胞之间的相互作用都有影响。
