Nielsen H B, Ahring B K
Bioprocess Science and Technology, BioCentrum, Building 227, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Environ Technol. 2007 Aug;28(8):905-14. doi: 10.1080/095933328086188485.
Two thermophilic continuously stirred tank reactors, R1 and R2, were subject to pulses of tryptone and ammonia. R1 was operated at an ammonia-N concentration of 3.0 g l(-1) and R2 was operated at an ammonia-N concentration of 1.7 g l(-1). Shock loads of tryptone (10 g l(-1), 10 g l(-1), 15 g l(-1)) had an immediate stimulating effect on methanogenesis for both reactors illustrated by significant peaks in methane production but also led to an organic overloading illustrated by a steep increase in volatile fatty acids (VFA) concentration. Three days after the pulses a second peak in acetate concentration and a decrease in methane production indicated an ammonia-inhibition of the acetoclastic methanogens. During the pulses of tryptone the performance of R1 was slightly more affected than R2. Pulses of ammonia (0.79 g l(-1) as N) resulted in a decrease in methane production of both reactors but no immediate increases in VFA concentrations was observed illustrating that the ammonia inhibition during this experiment was an overall inhibition of the biogas process and not only an inhibition of the methanogens.
两个嗜热连续搅拌釜式反应器R1和R2，接受了胰蛋白胨和氨的脉冲。R1在氨氮浓度为3.0 g l(-1)的条件下运行，R2在氨氮浓度为1.7 g l(-1)的条件下运行。胰蛋白胨的冲击负荷（10 g l(-1)、10 g l(-1)、15 g l(-1)）对两个反应器的甲烷生成均有即时刺激作用，表现为甲烷产量显著峰值，但也导致了有机负荷过载，表现为挥发性脂肪酸（VFA）浓度急剧增加。脉冲三天后，乙酸盐浓度出现第二个峰值，甲烷产量下降，表明氨对乙酸裂解产甲烷菌有抑制作用。在胰蛋白胨脉冲期间，R1的性能比R2受到的影响稍大。氨脉冲（0.79 g l(-1)作为N）导致两个反应器的甲烷产量下降，但未观察到VFA浓度立即增加，这表明该实验中的氨抑制是对沼气过程的整体抑制，而不仅仅是对产甲烷菌的抑制。
