Garcia Fernando U, Haber Marian M, Chen Xiaoli
Department of Pathology and Laboratory Medicine, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
Prostate. 2007 Nov 1;67(15):1686-92. doi: 10.1002/pros.20658.
Benign prostatic hyperplasia and prostatic adenocarcinoma exhibit prominent zonal predilections. Basal cells from the transitional zone and from the peripheral zone are postulated to have different underlying biological properties. We studied basal cells in both prostatic zones.
Tissue microarrays (TMA) were prepared from 65 whole-mounted prostatectomy specimens with prostatic adenocarcinoma. The transitional zone and peripheral zone were sampled from each prostate. TMA sections were stained with a basal cell cocktail (CK 34betaE12 + p63). The immunostaining pattern and the morphology of basal cells were recorded.
Triangular-shaped basal cells were highlighted by CK 34betaE12 cytoplasmic and p63 nuclear staining. These basal cells had their long axis oriented perpendicular to the basement membrane and their apex toward the lumen interdigited between secretory luminal cells. This morphology was seen in the majority of peripheral zone benign prostatic glands (92.0%) but only a minority of transitional zone benign prostatic glands (18.0%). Basal cells of the transitional zone showed weak or absent CK 34betaE12 staining in 65.9% of glands while maintaining p63. All glands with high-grade prostatic intraepithelial neoplasia (HGPIN) contained the triangular basal cells. In addition, basal cell clusters were identified in 8.7% of peripheral zone glands and 5.2% of HGPIN glands.
Our results indicate that the basal cell morphology and the basal cell immunophenotype have a zonal variation. The finding of a unique morphology of basal cells and the presence of basal cell clusters in the peripheral zone suggests that the peripheral zone might be the stem/progenitor cell-rich area in the human prostates.
良性前列腺增生和前列腺腺癌表现出明显的区域偏好性。推测来自移行区和外周区的基底细胞具有不同的潜在生物学特性。我们研究了前列腺两个区域的基底细胞。
从65例患有前列腺腺癌的完整前列腺切除标本制备组织微阵列（TMA）。从每个前列腺中采集移行区和外周区样本。TMA切片用基底细胞混合抗体（CK 34βE12 + p63）染色。记录基底细胞的免疫染色模式和形态。
CK 34βE12胞质和p63核染色突出显示了三角形基底细胞。这些基底细胞的长轴垂直于基底膜，其顶端朝向分泌性管腔细胞之间的管腔相互交错。这种形态在大多数外周区良性前列腺腺体内可见（92.0%），但在移行区良性前列腺腺体内仅少数可见（18.0%）。移行区的基底细胞在65.9%的腺体内显示CK 34βE12染色弱阳性或无染色，同时保持p63染色。所有伴有高级别前列腺上皮内瘤变（HGPIN）的腺体均含有三角形基底细胞。此外，在外周区腺体的8.7%和HGPIN腺体的5.2%中发现了基底细胞簇。
我们的结果表明基底细胞形态和基底细胞免疫表型存在区域差异。外周区基底细胞独特形态的发现以及基底细胞簇的存在表明外周区可能是人类前列腺中富含干/祖细胞的区域。
