基于芴甲氧羰基（Fmoc）的固相化学合成71聚体神经调节蛋白1-β1，一种表皮生长因子样结构域。

Fmoc-based solid phase chemical synthesis of 71-meric neuregulin 1-beta1, an epidermal growth factor-like domain.

作者信息

Kakizawa Taeko, Koide-Yoshida Shizuyo, Kimura Tooru, Uchimura Hiromasa, Hayashi Yoshio, Saito Kazuki, Kiso Yoshiaki

机构信息

Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century COE Program, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8412, Japan.

出版信息

J Pept Sci. 2008 Mar;14(3):261-6. doi: 10.1002/psc.916.

DOI:10.1002/psc.916

PMID:17880034

Abstract

The human neuregulin 1-beta1 (NRG1-beta1, amino acid residues 176-246) was chemically synthesized by Fmoc-based solid phase peptide synthesis (SPPS) followed by folding in a redox buffer. The biological activity of the synthesized NRG1-beta1 was confirmed by ligand-induced tyrosine phosphorylation on Chinese hamster ovary (CHO) cells expressing ErbB-4.

摘要

人神经调节蛋白1-β1（NRG1-β1，氨基酸残基176 - 246）通过基于Fmoc的固相肽合成（SPPS）进行化学合成，随后在氧化还原缓冲液中折叠。合成的NRG1-β1的生物活性通过在表达ErbB-4的中国仓鼠卵巢（CHO）细胞上配体诱导的酪氨酸磷酸化得到证实。