Nakagishi Yoshinori, Morimoto Yuji, Fujita Masanori, Ozeki Yuichi, Maehara Tadaaki, Kikuchi Makoto
Department of Medical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan.
Photochem Photobiol. 2007 Sep-Oct;83(5):1220-5. doi: 10.1111/j.1751-1097.2007.00148.x.
Airway stenosis in childhood is resistant to conventional treatments. Endoscope-assisted photodynamic therapy (PDT) is a potent candidate for the therapeutic modality owing to the easy approach to the tracheal lesion and low degree of invasiveness. The aim of the present study was to examine whether a photosensitizer preferentially accumulates in the lesion of airway stenosis in order to explore the possible applicability of PDT. The tracheal mucosa of rabbits was scraped off, and the rabbits were intravenously administered with Photofrin. The tissue concentration of Photofrin was quantitatively measured by fluorometric analysis. Granulation formation was seen in the mucosa-deprived lesion, causing airway stenosis. Photofrin concentration in the granulation tissue was four-fold higher than that in the intact trachea and 10-fold higher than that in the liver, spleen, skin and muscle. Photofrin preferentially accumulated in the lesion of airway stenosis. A preliminary experiment on PDT using transtracheal illumination showed an amelioration of airway stenosis, resulting in reduction in respiratory stridor.
儿童气道狭窄对传统治疗方法具有抗性。由于易于接近气管病变且侵袭程度低，内镜辅助光动力疗法（PDT）是一种有力的治疗方式候选方法。本研究的目的是检查光敏剂是否优先在气道狭窄病变中蓄积，以探索PDT的可能适用性。刮除兔的气管黏膜，然后给兔静脉注射血卟啉衍生物（Photofrin）。通过荧光分析定量测定血卟啉衍生物的组织浓度。在黏膜缺失病变处可见肉芽组织形成，导致气道狭窄。肉芽组织中的血卟啉衍生物浓度比完整气管中的高四倍，比肝脏、脾脏、皮肤和肌肉中的高十倍。血卟啉衍生物优先蓄积在气道狭窄病变中。使用经气管照明进行的PDT初步实验显示气道狭窄有所改善，呼吸喘鸣减轻。
