Matà S, Corzani S, Biagiotti R, Piacentini S, Siracusa G, Giudizi M G, Mastio M D, Borsini W, Taiuti R, Vultaggio A, Sorbi S, Maggi E
Department of Neurological and Psychiatric Sciences, Section of Immunoallergology and Respiratory Disease, University of Firenze, Firenze, Italy.
Eur J Neurol. 2007 Oct;14(10):1147-53. doi: 10.1111/j.1468-1331.2007.01929.x.
Autoimmune mechanisms are postulated to play a role in the development and progression of dysimmune neuropathies (DN). We investigated the relation between lymphocyte number and marker expression, and disease activity in 20 patients with DN under intravenous immunoglobulins (IVIg) treatment. B- and T-lymphocyte markers were studied by flow cytometry of the expression of CD5, CD25, CD23 and CD38 markers on B cells and of CD3, CD4 and CD8 markers, respectively. These parameters were compared with those obtained from matched healthy volunteers. The proportions of CD38+ B cells were higher in patients compared with those of controls. Proportions of activated CD4+ and CD8+ T cells were comparable in peripheral blood mononuclear cells of patients and controls, but a significant reduction of the absolute numbers of CD3+, CD4+ and CD8+ cells were observed in DN patients. The percentages of CD25+ memory T cells were instead significantly increased in DN patients. Lastly, T-cell reduction and the CD19/CD38 ratio over total B (CD19+) cells directly correlated with a poor response to IVIg therapy. In DN, whereas T-cell number is reduced, activated T and B cells are increased, thus suggesting an intrinsic defect of the immune response.
自身免疫机制被认为在免疫性神经病（DN）的发生和发展中起作用。我们研究了20例接受静脉注射免疫球蛋白（IVIg）治疗的DN患者的淋巴细胞数量与标志物表达及疾病活动之间的关系。通过流式细胞术分别研究B细胞上CD5、CD25、CD23和CD38标志物以及T细胞上CD3、CD4和CD8标志物的表达，以分析B淋巴细胞和T淋巴细胞标志物。将这些参数与匹配的健康志愿者所获得的参数进行比较。与对照组相比，患者中CD38 + B细胞的比例更高。患者和对照组外周血单个核细胞中活化的CD4 +和CD8 + T细胞比例相当，但DN患者中CD3 +、CD4 +和CD8 +细胞的绝对数量显著减少。相反，DN患者中CD25 +记忆T细胞的百分比显著增加。最后，T细胞减少以及总B（CD19 +）细胞上的CD19/CD38比值与IVIg治疗反应不佳直接相关。在DN中，虽然T细胞数量减少，但活化的T细胞和B细胞增加，因此提示免疫反应存在内在缺陷。
