Accardo-Palumbo A, Ferrante A, Ciccia F, Cadelo M, Giardina A R, Impastato R, Triolo G
Department of Internal Medicine, Division of Rheumatology, University of Palermo, Italy.
Int J Immunopathol Pharmacol. 2007 Jul-Sep;20(3):601-6. doi: 10.1177/039463200702000318.
The aim of this study is to evaluate the in vitro effect of pentoxifylline (PTX) on T Vgamma9/Vdelta2 lymphocyte function in Behçets disease (BD). We investigated the effect of PTX on Vgamma9/Vdelta2 T cell expansion and expression of TNFRII receptor and perforin content before and after PTX addition by means of FACS analysis lymphocyte cultures from patients with active and inactive BD and healthy subjects. The addition of PTX at a concentration of 1 mg/ml determined a significant inhibition of cell expansion, a down regulation of TNF receptor expression and inhibited the PMA-induced degranulation of perforin. Taken together these data indicate that PTX is capable of interfering with Vgamma9/Vdelta2 T cell function in BD, and although cell culture models cannot reliably predict all of the potential effects of the drug in vivo, our results encourage the possibility that this drug may find use in a range of immunological disorder characterized by dysregulated cell-mediated immunity.
本研究的目的是评估己酮可可碱（PTX）对贝赫切特病（BD）中Tγ9/δ2淋巴细胞功能的体外作用。我们通过流式细胞术分析来自活动期和非活动期BD患者及健康受试者的淋巴细胞培养物，研究了PTX添加前后对γ9/δ2 T细胞扩增、TNFRII受体表达和穿孔素含量的影响。添加浓度为1mg/ml的PTX可显著抑制细胞扩增，下调TNF受体表达，并抑制佛波酯（PMA）诱导的穿孔素脱颗粒。综合这些数据表明，PTX能够干扰BD中γ9/δ2 T细胞功能，尽管细胞培养模型不能可靠地预测该药物在体内的所有潜在作用，但我们的结果提示该药物可能在一系列以细胞介导免疫失调为特征的免疫性疾病中有用。
