Erata Gül Ozdemirler, Koçak Toker Necla, Durlanik Ozgür, Kadioğlu Ateş, Aktan Gülşen, Aykaç Toker Gülçin
Department of Biochemistry, Istanbul Medical Faculty, University of Istanbul, Capa, Istanbul, Turkey.
Fertil Steril. 2008 Aug;90(2):322-7. doi: 10.1016/j.fertnstert.2007.06.021. Epub 2007 Sep 19.
To clarify the role of heat shock protein 70 (Hsp 70) and its relation with DNA damage in male infertility.
Prospective study.
Andrology laboratory of Istanbul Medical Faculty.
PATIENT(S): Semen samples from 37 infertile men and 13 fertile men (as controls).
INTERVENTION(S): The percentage of DNA fragmentation was assayed with the use of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Sperm Hsp 70 expression was determined by using Western blot analysis.
MAIN OUTCOME MEASURE(S): Both the percentages of sperm DNA fragmentation and Hsp 70 expression were correlated with semen analysis parameters.
TUNEL-positive spermatozoa in the infertile group (18.7% for asthenospermics and 13.0% for oligoasthenospermics) were higher than the fertile group (4.9%). Significant inverse correlations were detected between percentage of TUNEL-positive cells and both concentration (r = -0.487) and motility (r = -0.377) of spermatozoa. No expression of Hsp 70 was observed in azospermic group, whereas Hsp 70 levels were found increased significantly in infertile group (U = 62 for asthenospermics and U = 38 for oligoasthenospermics) compared to fertile group as analyzed by using Mann-Whitney U Wilcoxon rank sum test. Furthermore, significant positive correlation was found between percentage of TUNEL-positive cells and Hsp 70 expression (r = 0.357).
CONCLUSION(S): Hsp 70 expression may have been increased as a protective mechanism against apoptosis in spermatozoa of infertile men.
阐明热休克蛋白70（Hsp 70）的作用及其与男性不育中DNA损伤的关系。
前瞻性研究。
伊斯坦布尔医学院男科实验室。
37名不育男性和13名生育男性（作为对照）的精液样本。
采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）检测DNA片段化百分比。通过蛋白质免疫印迹分析确定精子Hsp 70表达。
精子DNA片段化百分比和Hsp 70表达均与精液分析参数相关。
不育组中TUNEL阳性精子（弱精子症患者为18.7%，少弱精子症患者为13.0%）高于生育组（4.9%）。TUNEL阳性细胞百分比与精子浓度（r = -0.487）和活力（r = -0.377）之间均检测到显著负相关。无精子症组未观察到Hsp 70表达，而通过Mann-Whitney U Wilcoxon秩和检验分析发现，与生育组相比，不育组Hsp 70水平显著升高（弱精子症患者U = 62，少弱精子症患者U = 38）。此外，TUNEL阳性细胞百分比与Hsp 70表达之间存在显著正相关（r = 0.357）。
Hsp 70表达可能作为一种保护机制，在不育男性精子中对抗细胞凋亡而增加。
