Avendaño Conrado, Franchi Anahí, Taylor Steven, Morshedi Mahmood, Bocca Silvina, Oehninger Sergio
Department of Obstetrics and Gynecology, Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk, Virginia.
Fertil Steril. 2009 Apr;91(4):1077-84. doi: 10.1016/j.fertnstert.2008.01.015. Epub 2008 Apr 28.
To evaluate DNA fragmentation in spermatozoa with normal morphological appearance.
Prospective study.
Academic tertiary center.
PATIENT(S): Fertile, subfertile, and infertile men were studied.
INTERVENTION(S): Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-fluorescein nick-end labeling assay and morphology assessment by phase contrast in the swim-up fractions.
MAIN OUTCOME MEASURE(S): Simultaneous assessment of the percentage of normally shaped sperm and DNA fragmentation.
RESULT(S): No DNA fragmentation was found in spermatozoa with normal morphology in any of the samples from the fertile group. In only one sample from the subfertile group did we observed normally shaped sperm cells exhibiting DNA fragmentation. However, in all the samples from the infertile group, we observed normal spermatozoa with DNA fragmentation. Spermatozoa from this late group exhibited a high proportion of DNA damage.
CONCLUSION(S): In infertile men with moderate and severe teratozoospermia, the spermatozoa with apparently normal morphology present in the motile fractions after swim-up may have DNA fragmentation.
评估形态正常的精子中的DNA片段化情况。
前瞻性研究。
学术性三级中心。
对生育力正常、亚生育力和不育男性进行研究。
采用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸荧光素缺口末端标记法，并通过相差显微镜对上游组分进行形态学评估。
同时评估正常形态精子的百分比和DNA片段化情况。
在生育力正常组的任何样本中，形态正常的精子均未发现DNA片段化。在亚生育力组的仅一个样本中，我们观察到形态正常的精子细胞存在DNA片段化。然而，在不育组的所有样本中，我们均观察到存在DNA片段化的正常精子。该晚期组的精子表现出高比例的DNA损伤。
在患有中度和重度畸形精子症的不育男性中，上游后活动组分中形态明显正常的精子可能存在DNA片段化。
