Department of Reproductive Medicine, Shandong Research Institute for Family Planning, Jinan, China.
Fertil Steril. 2010 Aug;94(3):1027-32. doi: 10.1016/j.fertnstert.2009.04.034. Epub 2009 Jun 7.
To compare sperm chromatin dispersion (SCD) test with terminal deoxynucleotidyle transferase-mediated terminal uridine nick-end labeling (TUNEL) assay in assessing DNA fragmentation in human sperm through bright-field microscopy.
Prospectively designed, side-by-side comparative study.
Medical genetics laboratory in a provincial research institution.
PATIENT(S): Sixty male patients presented for infertility evaluation and 30 fertile, volunteer sperm donors.
INTERVENTION(S): Semen analysis, SCD test, and TUNEL assay on the same semen sample and on the same spermatozoa.
MAIN OUTCOME MEASURE(S): Sperm DNA fragmentation, determined by SCD test score or TUNEL assay score.
RESULT(S): Sperm chromatin dispersion test and TUNEL assay identified similar proportions of sperm cells with DNA fragmentation in the same semen samples. When the SCD test and TUNEL assay were performed simultaneously on the same spermatozoa, TUNEL-negative sperm showed a large halo, whereas TUNEL-positive sperm showed no halo after Diff-Quik staining. However, in some sperm cells, DNA damage was detected by the SCD test but not by the TUNEL assay.
CONCLUSION(S): Sperm chromatin dispersion test and TUNEL assay are both effective in detecting sperm DNA damage. Using bright-field microscopy, the SCD test appears to be more sensitive than the TUNEL assay.
通过明场显微镜比较精子染色质扩散（SCD）试验与末端脱氧核苷酸转移酶介导的末端尿嘧啶核苷尼克末端标记（TUNEL）检测法评估人精子 DNA 碎片化的效果。
前瞻性设计，并列对比研究。
省级研究机构的医学遗传学实验室。
60 名男性不育患者和 30 名健康、志愿捐精者。
对同一精液样本和同一精子进行精液分析、SCD 试验和 TUNEL 检测。
SCD 试验评分或 TUNEL 试验评分确定的精子 DNA 碎片化程度。
SCD 试验和 TUNEL 检测法在同一精液样本中识别出具有相似比例 DNA 碎片化的精子细胞。当同时在同一精子上进行 SCD 试验和 TUNEL 检测法时，TUNEL 阴性精子显示出较大的光晕，而 TUNEL 阳性精子在 Diff-Quik 染色后没有光晕。然而，在一些精子细胞中，SCD 试验检测到 DNA 损伤，但 TUNEL 检测法未检测到。
SCD 试验和 TUNEL 检测法均能有效检测精子 DNA 损伤。使用明场显微镜，SCD 试验似乎比 TUNEL 检测法更敏感。
