Tiwari Aseem Kumar, Shah Priti, Harimoorthy V
Prathama Blood Centre, Dr. C.V. Raman Marg, B/h Jivraj Mehta Hospital, Vasna, Ahmedabad.
Indian J Pathol Microbiol. 2007 Apr;50(2):437-40.
Red cell units issued to patient and returned back unused, are visually inspected for hemolysis before releasing them for reissue. A study was conducted to find out effect of temperature interruptions in the red cell units, during transport. The aims were to find out hemolysis in units with temperature interruptions and its relationship to the duration and also to compare actual hemolysis with visible hemolysis, if any. Thirty samples with different duration of temperature interruptions were evaluated for actual hemolysis. Fifteen samples of similar shelf-age but without temperature interruptions were included in the study as controls. Low hemoglobin photometer was used to measure supernatant hemoglobin. Despite temperature interruptions, almost all red cell units had acceptable level of hemolysis and this was similar to the red cell units without temperature interruption. The difference between the units with or without temperature interruption was not statistically significant (p > 0.05). Red cell units with smaller duration of temperature interruptions had lesser hemolysis than in those with longer temperature interruptions (p < 0.05). Red cell units younger than 7 days had lesser hemolysis than the units older than 8 days in both categories of units with and without temperature interruptions (p < 0.05). The units without visible hemolysis had lower hemolysis than units with visible hemolysis (p < 0.05). It can be concluded that temperature interruptions have a very minor role in hemolysis, younger units have lesser hemolysis than older units and visual assessment is subjective and tend to overestimate hemolysis.
发放给患者但未使用而退回的红细胞单位，在重新发放前需进行肉眼检查有无溶血情况。开展了一项研究，以查明红细胞单位在运输过程中温度中断的影响。目的是找出有温度中断的单位中的溶血情况及其与持续时间的关系，并比较实际溶血情况与可见溶血情况（如有）。对30个温度中断持续时间不同的样本进行了实际溶血评估。15个货架期相似但无温度中断的样本作为对照纳入研究。使用低血红蛋白光度计测量上清液血红蛋白。尽管存在温度中断，但几乎所有红细胞单位的溶血水平都可接受，这与无温度中断的红细胞单位相似。有或无温度中断的单位之间的差异无统计学意义（p>0.05）。温度中断持续时间较短的红细胞单位的溶血程度低于温度中断持续时间较长的单位（p<0.05）。在有和无温度中断的两类单位中，7天以内的红细胞单位的溶血程度低于8天以上的单位（p<0.05）。无可见溶血的单位的溶血程度低于有可见溶血的单位（p<0.05）。可以得出结论，温度中断在溶血中作用极小，较年轻的单位溶血程度低于较老的单位，且肉眼评估具有主观性，往往会高估溶血情况。
