Janatpour K A, Paglieroni T G, Crocker V L, DuBois D J, Holland P V
BloodSource and the University of California Davis Medical Center, Sacramento, California, USA.
Transfusion. 2004 Jul;44(7):984-9. doi: 10.1111/j.1537-2995.2004.03315.x.
Blood components that appear hemolyzed are discarded. However, visual inspection is subjective and criteria for excessive hemolysis are poorly defined.
Packed RBCs (10 CPDA-1, 10 Adsol) were collected. Half of each unit was leukoreduced. Plasma Hb was measured and compared in segments and units by three methods: 1) a HemoCue Plasma/Low Hb Photometer system; 2) a tetramethyl-benzidine (TMB) chemical method, and 3) a free Hb visual comparator.
Visual assessment tended to overestimate hemolysis. Chemical methods were comparable (r(2)= 0.894; HemoCue = 0.043 +[0.770]x TMB; n = 400; range, 0.01-0.5 g/dL), although the mean plasma Hb (g/dL) for the HemoCue method was higher than that of the TMB method (0.12 vs. 0.10 g/dL, respectively; p < 0.001). No units would have been discarded based on a hemolysis level of at least 0.6 g/dL (approx. 1%) if measured by a chemical method. However, 50 percent of CPDA-1 and 10 percent of Adsol units would have been discarded if only visual criteria were used. Leukoreduction did not increase plasma Hb levels. Discrepancies in plasma Hb levels were noted between units and their corresponding segments.
Visual assessment of hemolysis can result in unnecessary wastage of blood components. HemoCue offers an alternative, objective method to assess plasma Hb in the setting of blood collection and processing facilities for routine quality control and process validation, and may aid in the development of objective criteria for excessive hemolysis in blood components.
出现溶血的血液成分会被丢弃。然而，目视检查具有主观性，且对过度溶血的标准定义不明确。
收集了浓缩红细胞（10袋CPDA - 1、10袋Adsol）。每个单位的一半进行了白细胞滤除。通过三种方法测量并比较了血浆血红蛋白在各段及各单位中的含量：1）HemoCue血浆/低血红蛋白光度计系统；2）四甲基联苯胺（TMB）化学方法；3）游离血红蛋白目视比较仪。
目视评估往往高估溶血程度。化学方法具有可比性（r² = 0.894；HemoCue = 0.043 + [0.770]×TMB；n = 400；范围为0.01 - 0.5 g/dL），尽管HemoCue方法的平均血浆血红蛋白（g/dL）高于TMB方法（分别为0.12 vs. 0.10 g/dL；p < 0.001）。如果通过化学方法测量，基于至少0.6 g/dL（约1%）的溶血水平，没有单位会被丢弃。然而，如果仅使用目视标准，50%的CPDA - 1单位和10%的Adsol单位会被丢弃。白细胞滤除并未增加血浆血红蛋白水平。各单位与其相应段之间的血浆血红蛋白水平存在差异。
对溶血的目视评估可能导致血液成分的不必要浪费。HemoCue提供了一种替代的客观方法，用于在采血和处理设施中评估血浆血红蛋白，以进行常规质量控制和工艺验证，并且可能有助于制定血液成分过度溶血的客观标准。
