苯丙胺和可卡因对Lewis大鼠急性实验性过敏性脑脊髓炎发展的影响。

Effects of amphetamine and cocaine on the development of acute experimental allergic encephalomyelitis in Lewis rats.

作者信息

Núñez María J, Balboa José, Rey-Méndez Manuel, Brenlla Julio, González-Peteiro Mercedes, Rodrigo Elena, Freire-Garabal Manuel

机构信息

Neuroimmunology Laboratory, Department of Pharmacology, School of Medicine, Dentistry and Nursing, C/San Francisco, s/n. 15782 Santiago de Compostela, A Coruña, Spain.

出版信息

Hum Exp Toxicol. 2007 Aug;26(8):637-43. doi: 10.1177/0960327107076323.

DOI:10.1177/0960327107076323

PMID:17884951

Abstract

The present experiment deals with the effects of amphetamine and cocaine on the development and course of experimental allergic encephalomyelitis (EAE) induced in Lewis rats. Rats were immunized at the age of eight weeks with purified myelin basic protein isolated from guinea pig brain in complete Freund's adjuvant. Drug administration and recording of EAE clinical signs was performed daily since day 1 post-immunization (PI). On day 14 and 28 PI, six rats per group were bled and sacrificed. Spinal cord was examined histologically for EAE lesions. In vivo administration of 0.5 and 1 mg/Kg of amphetamine or cocaine resulted in a dose-related enhancement of neurological and histological signs of acute EAE in comparison with control rats. Both drugs caused a reduction of latent period together with a delayed regression of neurological signs along with an increase in inflammation in the central nervous system in comparison with placebo. Human & Experimental Toxicology (2007) 26, 637-643.

摘要

本实验研究了苯丙胺和可卡因对Lewis大鼠实验性变态反应性脑脊髓炎（EAE）的发生发展过程的影响。8周龄的大鼠用从豚鼠脑分离的纯化髓鞘碱性蛋白与完全弗氏佐剂一起进行免疫。自免疫后第1天（PI）起每天进行药物给药并记录EAE临床症状。在免疫后第14天和28天，每组处死6只大鼠并取血。对脊髓进行组织学检查以观察EAE病变。与对照大鼠相比，腹腔注射0.5和1mg/Kg的苯丙胺或可卡因导致急性EAE的神经学和组织学症状呈剂量依赖性增强。与安慰剂相比，两种药物均使潜伏期缩短，神经症状消退延迟，同时中枢神经系统炎症增加。《人类与实验毒理学》（2007年）26卷，637 - 643页。