Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, South Korea.
Phytother Res. 2010 Mar;24(3):399-403. doi: 10.1002/ptr.2959.
We examined whether fucoidan affected the clinical symptoms of experimental autoimmune encephalomyelitis (EAE) in rats. EAE was induced in Lewis rats that were immunized with guinea-pig myelin basic protein (MBP) and complete Freund's adjuvant. Fucoidan (50 mg/kg, daily) was administered to rats with EAE intraperitoneally, either in the EAE induction phase from either 1 day before immunization to day 7 post-immunization (PI), or the effector phase from day 8 to 14 PI, to test which phase of rat EAE is affected by fucoidan treatment.The onset, severity and duration of EAE paralysis in the fucoidan-treated group in the days 8-14 PI-treated rats, but not in days -1-7 PI-treated rats, were significantly delayed, suppressed and reduced, respectively, compared with the vehicle-treated controls. Treatment with fucoidan reduced the encephalitogenic response and TNF-alpha production during EAE. Moreover, the clinical amelioration coincided with decreased infiltration of inflammatory cells in the EAE-affected spinal cord. The ameliorative effect of fucoidan on clinical paralysis in EAE-affected rats may be mediated, in part, by the suppression of the autoreactive T cell response and inflammatory cytokine production.
我们研究了岩藻聚糖硫酸酯是否会影响实验性自身免疫性脑脊髓炎（EAE）大鼠的临床症状。通过用豚鼠髓鞘碱性蛋白（MBP）和完全弗氏佐剂免疫Lewis 大鼠诱导 EAE。岩藻聚糖硫酸酯（50mg/kg，每日）通过腹腔内给药给患有 EAE 的大鼠，要么在免疫前 1 天至免疫后 7 天（PI）的诱导期内，要么在 8 至 14 PI 的效应期内，以测试岩藻聚糖硫酸酯治疗会影响大鼠 EAE 的哪个阶段。与载体处理对照组相比，在 8-14 PI 治疗的大鼠中，岩藻聚糖硫酸酯治疗组的 EAE 瘫痪的发作、严重程度和持续时间分别显著延迟、抑制和减少。岩藻聚糖硫酸酯治疗降低了 EAE 期间的致脑炎反应和 TNF-α 产生。此外，临床改善与 EAE 受累脊髓中炎症细胞浸润的减少相吻合。岩藻聚糖硫酸酯对 EAE 受累大鼠临床瘫痪的改善作用可能部分是通过抑制自身反应性 T 细胞反应和炎症细胞因子的产生来介导的。
