Garbers Eike, Mitlöhner Rita, Georgieva Radostina, Bäumler Hans
Institute of Transfusion Medicine, Charité-Universitätsmedizin Berlin, D-10098 Berlin, Germany.
Macromol Biosci. 2007 Dec 6;7(12):1243-9. doi: 10.1002/mabi.200700117.
Polyelectrolyte microcapsules composed by using the LbL technique on stabilized RBC as templates were coated with up to ten layer pairs of trypsin/PSS or trypsin/alginate. The trypsin layer growth was confirmed by particle electrophoresis, confocal laser scanning microscopy, flow cytometry, and protein determination according to Lowry. In the coating series with trypsin/PSS, the amount of immobilized enzyme was larger than that with trypsin/alginate. The enzyme immobilization led to activity reduction of up to 90% compared to that of the same enzyme amount in the solution. No significant differences between the activities of trypsin immobilized in combination with PSS and with alginate were found.
以稳定化红细胞为模板，采用层层自组装技术制备的聚电解质微胶囊，用多达十层的胰蛋白酶/聚磺苯乙烯或胰蛋白酶/海藻酸盐进行包被。通过颗粒电泳、共聚焦激光扫描显微镜、流式细胞术以及根据洛瑞法进行的蛋白质测定，证实了胰蛋白酶层的生长。在胰蛋白酶/聚磺苯乙烯的包被系列中，固定化酶的量大于胰蛋白酶/海藻酸盐包被系列。与溶液中相同酶量相比，酶固定化导致活性降低高达90%。未发现与聚磺苯乙烯和海藻酸盐结合固定化的胰蛋白酶活性之间存在显著差异。
