Bucko Marek, Gemeiner Peter, Vikartovská Alica, Mislovicová Danica, Lacík Igor, Tkác Ján
Department of Glycobiotechnology, Institute of Chemistry - Center for Glycomics, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Artif Cells Blood Substit Immobil Biotechnol. 2010 Apr;38(2):90-8. doi: 10.3109/10731191003634745.
A novel encapsulated oxidative biocatalyst comprising glucose oxidase (GOD) coencapsulated with oxygen carriers within polyelectrolyte complex capsules was developed for the production of D-gluconic acid and delta-gluconolactone. The capsules containing immobilized GOD were produced by polyelectrolyte complexation with sodium alginate (SA) and cellulose sulfate (CS) as polyanions, poly(methylene-co-guanidine) (PMCG) as the polycation, CaCl(2) as the gelling agent and NaCl as the antigelling agent (GOD-SA-CS/PMCG capsules). Poly(dimethylsiloxane) (PDMS) and an emulsion of n-dodecane (DOD) or perfluorodecaline (PFD) with PDMS were used as the oxygen carriers and MnO(2) was used as a hydrogen peroxide decomposition catalyst. Water-soluble PDMS was found to act as both an oxygen carrier and an emulsifier of water-insoluble DOD and PFD. Stable microcapsules could be produced with concentrations of up to 4% (w/w) of PDMS, 10% (w/w) of DOD and PFD, and 25% (w/w) of MnO(2) in the polyanion solution of SA and CS. Roughly a two-fold increase in the GOD activity from 21.0+/-1.1 to 38.4+/-2.0 Ug(-1) and product space-time yields (STY) from 44.3+/-2.0 to 83.4+/-3.4 gH*day(-1) could be achieved utilizing coencapsulated oxygen carriers compared to GOD encapsulated in the absence of oxygen carriers. This enhanced production does not significantly depend on the selected oxygen carrier under the conditions used in this study.
一种新型的包封式氧化生物催化剂被开发用于生产D - 葡萄糖酸和δ - 葡萄糖酸内酯，该催化剂由葡萄糖氧化酶（GOD）与氧载体共同包封在聚电解质复合胶囊中。含有固定化GOD的胶囊通过以海藻酸钠（SA）和硫酸纤维素（CS）为聚阴离子、聚（亚甲基 - 共 - 胍）（PMCG）为聚阳离子、氯化钙（CaCl₂）为胶凝剂以及氯化钠为抗胶凝剂（GOD - SA - CS/PMCG胶囊）进行聚电解质络合制备。聚二甲基硅氧烷（PDMS）以及正十二烷（DOD）或全氟萘烷（PFD）与PDMS的乳液用作氧载体，二氧化锰（MnO₂）用作过氧化氢分解催化剂。发现水溶性PDMS既作为氧载体又作为水不溶性DOD和PFD的乳化剂。在SA和CS的聚阴离子溶液中，当PDMS浓度高达4%（w/w）、DOD和PFD浓度高达10%（w/w）以及MnO₂浓度高达25%（w/w）时，可以制备出稳定的微胶囊。与未包封氧载体的GOD相比，利用共同包封的氧载体可使GOD活性从21.0±1.1单位·克⁻¹大致提高两倍至38.4±2.0单位·克⁻¹，产物时空产率（STY）从44.3±2.0克·小时⁻¹·天⁻¹提高至83.4±3.4克·小时⁻¹·天⁻¹。在本研究使用的条件下，这种产量的提高并不显著依赖于所选择的氧载体。
