Singh Ashok K, Jiang Yin, Benlhabib Elhabib, Gupta Shveta
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Twin Cities Campus, St. Paul, MN 55108, USA.
J Med Food. 2007 Sep;10(3):526-42. doi: 10.1089/jmf.2006.228.
Chronic alcohol drinking has been associated with the development of a number of abnormalities, including neuron-behavioral disorders, liver, pancreas, and heart-related diseases and inflammation and immune disorders. Because diverse mechanisms are involved in the development of these disorders, the commonly used receptor- or enzyme-specific drugs do not provide comprehensive protection against the adverse effects of alcoholism. This study describes possible therapeutic potency of puerarin (PU) from kudzu root, polyenylphosphatidylcholine from soy (SPCh), and curcumin (CU) from turmeric against alcohol's addiction-related and inflammatory-related abnormalities in alcohol-preferring P rats receiving free choice water and 15% ethanol in water. P-rats were fed once daily either the vehicle (for control) or different doses of PU, SPCh, CU, PU + SPCh, or PU + CU. The rats were divided in two groups: one received water alone, and the other free choice water and ethanol. Four rats from each group were fitted with electroencephalogram (EEG) electrodes for EEG recording. After 70 days of alcohol drinking, alcohol was withdrawn for 2 weeks, and the withdrawal symptoms were assessed. This study showed that alcohol drinking for 70 days (1) caused liver inflammation characterized by elevated tumor necrosis factor-alpha, interleukin-1beta, and matrix metalloproteinase-9 expression and (2) dysregulated lipopolysaccharide (LPS)-induced pleurisy. Alcohol withdrawal after 70 days of drinking generated severe withdrawal symptoms including seizure-type EEG activity. PU suppressed the addiction-mediated abnormalities but did not affect the inflammation-related abnormalities, while SPCh or CU suppressed only the inflammation-related abnormalities in alcohol-drinking rats subjected to LPS-induced pleurisy. A combination of PU with SPCh or CU suppressed both the addiction-related and inflammation-related abnormalities of alcohol drinking. Therefore, a mixture consisting of PU and either SPCh or CU may provide alternative therapy for alcohol-related disorders.
长期饮酒与多种异常情况的发生有关，包括神经行为障碍、肝脏、胰腺和心脏相关疾病以及炎症和免疫紊乱。由于这些疾病的发生涉及多种机制，常用的受体或酶特异性药物并不能全面预防酒精中毒的不良影响。本研究描述了葛根中的葛根素（PU）、大豆中的多烯磷脂酰胆碱（SPCh）和姜黄中的姜黄素（CU）对偏好酒精的P大鼠在自由选择水和15%乙醇水溶液时与酒精成瘾及炎症相关异常的潜在治疗作用。给P大鼠每日一次喂食赋形剂（作为对照）或不同剂量的PU、SPCh、CU、PU + SPCh或PU + CU。大鼠分为两组：一组只饮水，另一组自由选择水和乙醇。每组四只大鼠安装脑电图（EEG）电极用于EEG记录。饮酒70天后，戒酒2周，并评估戒断症状。本研究表明，饮酒70天（1）导致肝脏炎症，表现为肿瘤坏死因子-α、白细胞介素-1β和基质金属蛋白酶-9表达升高，（2）使脂多糖（LPS）诱导的胸膜炎失调。饮酒70天后戒酒产生严重的戒断症状，包括癫痫样EEG活动。PU可抑制成瘾介导的异常，但不影响炎症相关异常，而SPCh或CU仅抑制LPS诱导胸膜炎的饮酒大鼠的炎症相关异常。PU与SPCh或CU联合使用可抑制饮酒的成瘾相关和炎症相关异常。因此，由PU与SPCh或CU组成的混合物可能为酒精相关疾病提供替代疗法。
