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水泡性口炎病毒的RNA温度敏感突变体：I组突变体的转录酶限制取决于L蛋白的热敏感性。

RNA- temperature-sensitive mutants of vesicular stomatitis virus: L-protein thermosensitivity accounts for transcriptase restriction of group I mutants.

作者信息

Hunt D M, Emerson S U, Wagner R R

出版信息

J Virol. 1976 May;18(2):596-603. doi: 10.1128/JVI.18.2.596-603.1976.

DOI:10.1128/JVI.18.2.596-603.1976
PMID:178900
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC515586/
Abstract

In vitro transcriptase activity of three group I temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 C was restored by L-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. Soluble NS protein from wt nucleocapsids did not reconstitute restricted transcriptions of the group I RNA-ts mutants. NS protein activity, but not L protein activity, was purified from the group I ts mutants; this NS fraction always displayed the wt phenotype in reconstitution assays. Neither the L nor the NS protein was capable of restoring the defective transcriptive activity of the group IV vesicular stomatitis virus mutant ts W16B.

摘要

在39℃受到限制的水泡性口炎病毒的三个I组温度敏感（ts）突变体的体外转录酶活性，可通过源自野生型（wt）水泡性口炎病毒粒子核衣壳的L蛋白组分恢复。来自wt核衣壳的可溶性NS蛋白不能重建I组RNA-ts突变体的受限转录。从I组ts突变体中纯化出了NS蛋白活性而非L蛋白活性；该NS组分在重组试验中始终表现出wt表型。L蛋白和NS蛋白均不能恢复IV组水泡性口炎病毒突变体ts W16B的缺陷转录活性。

相似文献

1
RNA- temperature-sensitive mutants of vesicular stomatitis virus: L-protein thermosensitivity accounts for transcriptase restriction of group I mutants.水泡性口炎病毒的RNA温度敏感突变体：I组突变体的转录酶限制取决于L蛋白的热敏感性。
J Virol. 1976 May;18(2):596-603. doi: 10.1128/JVI.18.2.596-603.1976.
2
Location of the transcription defect in group I temperature-sensitive mutants of vesicular stomatitis virus.水泡性口炎病毒I组温度敏感突变体中转录缺陷的定位
J Virol. 1974 Jan;13(1):28-35. doi: 10.1128/JVI.13.1.28-35.1974.
3
Study of the transcription and the replication of vesicular stomatitis virus by using temperature-sensitive mutants.利用温度敏感突变体研究水疱性口炎病毒的转录和复制
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Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus.水泡性口炎病毒在体外进行RNA合成需要NS蛋白和L蛋白。
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Temperature-sensitive mutants of vesicular stomatitis virus: comparison of the in vitro RNA polymerase defects of group I and group IV mutants.水泡性口炎病毒的温度敏感突变体：I组和IV组突变体的体外RNA聚合酶缺陷比较。
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The role of polypeptides L and NS in the transcription process of vesicular stomatitis virus New Jersey using the temperature-sensitive mutant tsE1.使用温度敏感突变体tsE1研究多肽L和NS在水疱性口炎病毒新泽西株转录过程中的作用。
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引用本文的文献

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Noncytopathic mutants of Newcastle disease virus are defective in virus-specific RNA synthesis.新城疫病毒的非细胞病变突变体在病毒特异性RNA合成方面存在缺陷。
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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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BIOLOGIC PROPERTIES OF TWO PLAQUE VARIANTS OF VESICULAR STOMATITIS VIRUS (INDIANA SEROTYPE).水泡性口炎病毒（印第安纳血清型）两种蚀斑变体的生物学特性
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Temperature-sensitive mutants of vesicular stomatitis virus: comparison of the in vitro RNA polymerase defects of group I and group IV mutants.水泡性口炎病毒的温度敏感突变体：I组和IV组突变体的体外RNA聚合酶缺陷比较。
J Virol. 1974 Oct;14(4):765-72. doi: 10.1128/JVI.14.4.765-772.1974.
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Reconstitution of infectivity and transcriptase activity of homologous and heterologous viruses: vesicular stomatitis (Indiana serotype), Chandipura, vesicular stomatitis (New Jersey serotype), and Cocal viruses.同源和异源病毒的感染性及转录酶活性重建：水疱性口炎病毒（印第安纳血清型）、钱德布尔病毒、水疱性口炎病毒（新泽西血清型）及科卡尔病毒
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Transcription and replication of vesicular stomatitis virus: effects of temperature-sensitive mutations in complementation group IV.水泡性口炎病毒的转录与复制：互补群IV中温度敏感突变的影响
J Virol. 1974 Apr;13(4):922-30. doi: 10.1128/JVI.13.4.922-930.1974.
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Location of the transcription defect in group I temperature-sensitive mutants of vesicular stomatitis virus.水泡性口炎病毒I组温度敏感突变体中转录缺陷的定位
J Virol. 1974 Jan;13(1):28-35. doi: 10.1128/JVI.13.1.28-35.1974.
7
RNA synthesis of vesicular stomatitis virus. V. Interactions between transcription and replication.水泡性口炎病毒的RNA合成。V.转录与复制之间的相互作用。
J Virol. 1973 Dec;12(6):1395-400. doi: 10.1128/JVI.12.6.1395-1400.1973.
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L protein requirement for in vitro RNA synthesis by vesicular stomatitis virus.水泡性口炎病毒体外RNA合成对L蛋白的需求。
J Virol. 1973 Dec;12(6):1325-35. doi: 10.1128/JVI.12.6.1325-1335.1973.
9
Primary in vivo transcription of vesicular stomatitis virus and temperature-sensitive mutants of five vesicular stomatitis virus complementation groups.水泡性口炎病毒及其五个互补组的温度敏感突变体的体内初级转录
J Virol. 1973 Dec;12(6):1238-52. doi: 10.1128/JVI.12.6.1238-1252.1973.
10
Temperature-sensitive mutants of vesicular stomatitis virus; homology and nomenclature.水泡性口炎病毒的温度敏感突变体；同源性与命名
J Gen Virol. 1973 Jun;19(3):295-300. doi: 10.1099/0022-1317-19-3-295.