水泡性口炎病毒的RNA温度敏感突变体：I组突变体的转录酶限制取决于L蛋白的热敏感性。
RNA- temperature-sensitive mutants of vesicular stomatitis virus: L-protein thermosensitivity accounts for transcriptase restriction of group I mutants.
作者信息
Hunt D M, Emerson S U, Wagner R R
出版信息
J Virol. 1976 May;18(2):596-603. doi: 10.1128/JVI.18.2.596-603.1976.
Abstract
In vitro transcriptase activity of three group I temperature-sensitive (ts) mutants of vesicular stomatitis virus restricted at 39 C was restored by L-protein fractions derived from wild-type (wt) vesicular stomatitis virion nucleo-capsids. Soluble NS protein from wt nucleocapsids did not reconstitute restricted transcriptions of the group I RNA-ts mutants. NS protein activity, but not L protein activity, was purified from the group I ts mutants; this NS fraction always displayed the wt phenotype in reconstitution assays. Neither the L nor the NS protein was capable of restoring the defective transcriptive activity of the group IV vesicular stomatitis virus mutant ts W16B.
摘要
在39℃受到限制的水泡性口炎病毒的三个I组温度敏感（ts）突变体的体外转录酶活性，可通过源自野生型（wt）水泡性口炎病毒粒子核衣壳的L蛋白组分恢复。来自wt核衣壳的可溶性NS蛋白不能重建I组RNA-ts突变体的受限转录。从I组ts突变体中纯化出了NS蛋白活性而非L蛋白活性；该NS组分在重组试验中始终表现出wt表型。L蛋白和NS蛋白均不能恢复IV组水泡性口炎病毒突变体ts W16B的缺陷转录活性。
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Temperature-sensitive mutants of vesicular stomatitis virus: comparison of the in vitro RNA polymerase defects of group I and group IV mutants.水泡性口炎病毒的温度敏感突变体：I组和IV组突变体的体外RNA聚合酶缺陷比较。
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