Tarcea Nicolae, Harz Michaela, Rösch Petra, Frosch Torsten, Schmitt Michael, Thiele Hans, Hochleitner R, Popp Jürgen
Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, D-07743 Jena, Germany.
Spectrochim Acta A Mol Biomol Spectrosc. 2007 Dec 15;68(4):1029-35. doi: 10.1016/j.saa.2007.06.051. Epub 2007 Aug 11.
We report on the great advantages of using deep UV Raman system for in situ planetary applications. Among them are to be mentioned: (I) higher scattering efficiency compared to VIS-IR Raman excitation wavelengths, (II) electronic resonance effects which increase the intrinsically weak Raman signal thus improving the S/N ratio of the detected Raman signals and (III) spectral separation of Raman and fluorescence signals. All these advantages are making UV Raman a valuable technique for in situ planetary applications. Mineral as well as biological samples were analyzed using Raman deep UV excitation and the results are presented. For the mineral samples a comparison with excitation in the NIR-VIS spectral regions is made. The impact of fluorescence on Raman data acquisition at different laser excitation wavelengths is assessed. Making use of the resonance effects, spectra of microorganisms were recorded with a high S/N ratio, allowing afterwards a very precise identification and classification (to the strain level) of the measured samples.
我们报告了使用深紫外拉曼系统进行原位行星应用的巨大优势。其中包括：（I）与可见-红外拉曼激发波长相比，散射效率更高；（II）电子共振效应，可增强本质上较弱的拉曼信号，从而提高检测到的拉曼信号的信噪比；（III）拉曼信号与荧光信号的光谱分离。所有这些优势使紫外拉曼成为原位行星应用的一项有价值的技术。使用拉曼深紫外激发对矿物和生物样品进行了分析，并展示了结果。对于矿物样品，与近红外-可见光谱区域的激发进行了比较。评估了荧光对不同激光激发波长下拉曼数据采集的影响。利用共振效应，以高信噪比记录了微生物的光谱，随后能够对测量样品进行非常精确的鉴定和分类（到菌株水平）。
