雅巴猴肿瘤病毒的蛋白质 I. 结构蛋白
Proteins of Yaba monkey tumor virus I. Structural proteins.
作者信息
Fenger T, Rouhandeh H
出版信息
J Virol. 1976 May;18(2):757-64. doi: 10.1128/JVI.18.2.757-764.1976.
Yaba virus proteins were characterized by polyacrylamide gel electrophoresis. Electrophoresis of Yaba virion (proteins) dissociated by sodium dodecyl sulfate and 2-mercaptoethanol in continuous and discontinuous buffer systems yielded 37 polypeptide species by staining and by counting bands of radioactively labeled polypeptides. The molecular weights of the viral polypeptide species were found to range from 10,000 to 220,000 by comparing the relative distance of migration of viral proteins with proteins of known molecular weights. Two polypeptides were removed from purified virions by nonionic detergent nonidet P -40 treatment, and the amount of one polypeptide was reduced. Purified cores yielded 21 polypeptide species, none of which was labeled with radioactive glucosamine.
雅巴病毒蛋白通过聚丙烯酰胺凝胶电泳进行表征。在连续和不连续缓冲系统中，用十二烷基硫酸钠和2-巯基乙醇解离的雅巴病毒粒子（蛋白）进行电泳，通过染色和计数放射性标记多肽的条带，得到了37种多肽。通过比较病毒蛋白与已知分子量蛋白的相对迁移距离，发现病毒多肽的分子量范围为10,000至220,000。用非离子去污剂诺乃得P -40处理从纯化的病毒粒子中去除了两种多肽，并且一种多肽的量减少了。纯化的核心产生了21种多肽，其中没有一种用放射性葡糖胺标记。
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