Sokol F, Stancek D, Koprowski H
J Virol. 1971 Feb;7(2):241-9. doi: 10.1128/JVI.7.2.241-249.1971.
Purified rabies virions, unlabeled or labeled with radioactive amino acids or d-glucosamine, were dissociated into their polypeptides by treatment with sodium dodecyl sulfate in a reducing environment and fractionated by electroiphoresis in sodium dodecyl sulfate-containing polyacrylamide gel. The molecular weights of individual polypeptides were estimated by comparison of their rate of migration with that of protein markers of known molecular weight. Purified viral nucleocapsid and a mixture of envelope components, isolated from virions disrupted by sodium deoxycholate, were analyzed by the same procedure. The number of molecules per virion of each polypeptide was estimated from the proportions of the separated components, the known molecular weight of the viral ribonucleic acid, and the chemical composition of the nucleocapsid. The protein moiety of the nucleocapsid particle was estimated to consist of 1,713 molecules of a major polypeptide (molecular weight, 62,000 daltons) and 76 molecules of a minor polypeptide (molecular weight, 55,000 daltons). In addition to 1,783 molecules of a glycoprotein component (molecular weight, 80,000 daltons), the viral envelope contains 789 and 1,661 molecules, respectively, of two other polypeptides (molecular weight, 40,000 and 25,000 daltons).
纯化的狂犬病病毒粒子，未标记或用放射性氨基酸或D-葡萄糖胺标记，在还原环境中用十二烷基硫酸钠处理，使其解离成多肽，并在含十二烷基硫酸钠的聚丙烯酰胺凝胶中进行电泳分离。通过将各个多肽的迁移速率与已知分子量的蛋白质标志物的迁移速率进行比较，来估计单个多肽的分子量。从被脱氧胆酸钠破坏的病毒粒子中分离出的纯化病毒核衣壳和包膜成分混合物，采用相同的程序进行分析。根据分离成分的比例、病毒核糖核酸的已知分子量以及核衣壳的化学组成，估计每个病毒粒子中每种多肽的分子数。核衣壳粒子的蛋白质部分估计由1713个主要多肽分子（分子量62000道尔顿）和76个次要多肽分子（分子量55000道尔顿）组成。除了1783个糖蛋白成分分子（分子量80000道尔顿）外，病毒包膜还分别含有789个和1661个另外两种多肽分子（分子量40000和25000道尔顿）。
