Tasdemir Ezgi, Maiuri M Chiara, Tajeddine Nicolas, Vitale Ilio, Criollo Alfredo, Vicencio José Miguel, Hickman John A, Geneste Olivier, Kroemer Guido
INSERM, Villejuif, France.
Cell Cycle. 2007 Sep 15;6(18):2263-7. doi: 10.4161/cc.6.18.4681. Epub 2007 Jul 2.
When added to cells, a variety of autophagy inducers that operate through distinct mechanisms and target different organelles for autophagic destruction (mitochondria in mitophagy, endoplasmic reticulum in reticulophagy) rarely induce autophagic vacuolization in more than 50% or the cells. Here we show that this heterogeneity may be explained by cell cycle-specific effects. The BH3 mimetic ABT737, lithium, rapamycin, tunicamycin or nutrient depletion stereotypically induce autophagy preferentially in the G(1) and S phases of the cell cycle, as determined by simultaneous monitoring of cell cycle markers and the cytoplasmic aggregation of GFP-LC3 in autophagic vacuoles. These results point to a hitherto neglected crosstalk between autophagic vacuolization and cell cycle regulation.
当添加到细胞中时，多种通过不同机制发挥作用并针对不同细胞器进行自噬性破坏（线粒体进行线粒体自噬，内质网进行网织红细胞自噬）的自噬诱导剂，很少能在超过50%的细胞中诱导自噬空泡化。我们在此表明，这种异质性可能由细胞周期特异性效应来解释。BH3模拟物ABT737、锂、雷帕霉素、衣霉素或营养物质耗竭通常优先在细胞周期的G(1)期和S期诱导自噬，这是通过同时监测细胞周期标志物和自噬空泡中GFP-LC3的细胞质聚集来确定的。这些结果表明自噬空泡化与细胞周期调控之间存在迄今被忽视的相互作用。
