甲状旁腺激素相关蛋白在体外抑制刺激引起的子宫收缩。

Parathyroid hormone-related protein inhibits stimulated uterine contraction in vitro.

作者信息

Shew R L, Yee J A, Kliewer D B, Keflemariam Y J, McNeill D L

机构信息

Department of Anatomical Sciences, Oklahoma University Health Sciences Center, Oklahoma City.

出版信息

J Bone Miner Res. 1991 Sep;6(9):955-9. doi: 10.1002/jbmr.5650060909.

DOI:10.1002/jbmr.5650060909

PMID:1789142

Abstract

The effect of parathyroid hormone-related protein (PTHrP) fragments 1-34, 38-64, and 67-86 on acetylcholine-stimulated rat uterine contraction was examined in vitro. In addition, the possibility that PTHrP-(38-64) or (67-86) influenced relaxation caused by PTHrP-(1-34) was also investigated. Contraction of uterine horns was stimulated with 10(-6) or 10(-5) M acetylcholine. PTHrP-(1-34) reduced the magnitude of acetylcholine-stimulated uterine contraction. This effect was dose related over a concentration range of 10(-9)-10(-6) M. Neither PTHrP-(38-64) or PTHrP-(67-86) at concentrations of 10(-8)-10(-6) M affected uterine contraction stimulated by 10(-6) M acetylcholine. These fragments did not affect the relaxation caused by 10(-7) M PTHrP-(1-34). These results demonstrate that (1) PTHrP-(1-34) at 10(-6) M influences contraction of the rat myometrium and (2) the muscle relaxant activity of PTHrP is associated with the first 34 N-terminal amino acids.

摘要

在体外研究了甲状旁腺激素相关蛋白（PTHrP）片段1 - 34、38 - 64和67 - 86对乙酰胆碱刺激的大鼠子宫收缩的影响。此外，还研究了PTHrP -（38 - 64）或（67 - 86）影响由PTHrP -（1 - 34）引起的舒张的可能性。用10^(-6)或10^(-5) M乙酰胆碱刺激子宫角收缩。PTHrP -（1 - 34）降低了乙酰胆碱刺激的子宫收缩幅度。在10^(-9) - 10^(-6) M的浓度范围内，这种作用与剂量相关。浓度为10^(-8) - 10^(-6) M的PTHrP -（38 - 64）或PTHrP -（67 - 86）均不影响由10^(-6) M乙酰胆碱刺激引起的子宫收缩。这些片段不影响由10^(-7) M PTHrP -（1 - 34）引起的舒张。这些结果表明：（1）10^(-6) M的PTHrP -（1 - 34）影响大鼠子宫肌层的收缩；（2）PTHrP的肌肉舒张活性与N端前34个氨基酸有关。