Yoshida Izumi, Isagawa Satoshi, Kibune Nobuyuki, Hamano-Nagaoka Megumi, Maitani Tamio
Japan Food Research Laboratories, 7-4-41 Asagi, Saito, Ibaraki-shi, Osaka 567-0085, Japan.
Shokuhin Eiseigaku Zasshi. 2007 Aug;48(4):83-9. doi: 10.3358/shokueishi.48.83.
Furan is a 5-membered ring compound with high volatility. The U.S. Food and Drug Administration (FDA) has recently published a report on the occurrence of furan in a large number of thermally processed foods. However, the FDA's analytical method, using standard curve addition, is not suitable for high-throughput routine laboratory operations. We developed a rapid and improved method for determination of furan in foods by headspace GC/MS. Quantification was achieved by using an internal standard of d4-furan and an external calibration curve of furan normalized against the internal standard. The incubation temperature for equilibration was set at 60 degrees C to avoid the formation of furan during analysis. The levels of furan in baby foods and infant formulas were determined with this method. Validation data showed good precision and accuracy. The LOD and LOQ were 0.2-0.5 ng/g and 0.5-2 ng/g for various food matrixes, respectively. The level of furan detected was in the range of 1.4 to 90 ng/g in baby foods and in the range of non-detectable to 36 ng/g in infant formulas.
呋喃是一种具有高挥发性的五元环化合物。美国食品药品监督管理局（FDA）最近发布了一份关于大量热加工食品中呋喃含量的报告。然而，FDA使用标准曲线加入法的分析方法并不适用于高通量的常规实验室操作。我们开发了一种通过顶空气相色谱/质谱法快速测定食品中呋喃含量的改进方法。通过使用d4-呋喃作为内标以及以该内标归一化的呋喃外标校准曲线来实现定量。平衡孵育温度设定为60摄氏度，以避免在分析过程中形成呋喃。用该方法测定了婴儿食品和婴儿配方奶粉中呋喃的含量。验证数据显示出良好的精密度和准确度。对于各种食品基质，检测限和定量限分别为0.2 - 0.5 ng/g和0.5 - 2 ng/g。在婴儿食品中检测到的呋喃含量范围为1.4至90 ng/g，在婴儿配方奶粉中为未检出至36 ng/g。
