Kikuvi G M, Ombui J N, Mitema E S, Schwarz S
Institute of Tropical Medicine and Infectious Diseases (ITROMID), Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya.
East Afr Med J. 2007 May;84(5):233-9. doi: 10.4314/eamj.v84i5.9531.
To isolate Salmonella from food animals and characterise the antimicrobial resistance of the isolates.
A random sampling of slaughter animals was carried out.
Department of Public Health, Pharmacology and Toxicology, University of Nairobi, Kenya and Institute for Animal Breeding, Neustadt-Mariensee, Germany.
Two hundred and eighty five samples, including faecal samples and carcass, cloacal and pharyngeal swab samples were analysed.
Sixteen (5.6%) of 285 samples were positive for Salmonella. The prevalence of Salmonella on pig carcasses (19%) was higher than in faeces (8.6%). Three Salmonella enterica sub-species enterica serovars, namely Saintpaul (S. Saintpaul), Braenderup (S. Braenderup), and Heidelberg (S. Heidelberg), were identified, with S. Saintpaul being the predominant serovar. Antimicrobial resistance was found in 35.7% of all the isolates. The S. Heidelberg isolates were susceptible to all the antimicrobial agents tested. Multidrug resistance was found in 7.1% of the resistant Salmonella isolates. Plasmids were only detected in S. Heidelberg. Ampicillin resistance was based on expression of a bla(TEM) gene, while chloramphenicol, streptomycin, and tetracycline resistances were encoded by the genes catAl, strA, and tet(A), respectively.
Pigs may serve as reservoirs of antimicrobial resistant Salmonella and slaughterhouse cross-contamination of pork may be a food safety risk. We recommended that slaughterhouse hygiene be improved to minimise contamination of pig carcasses.
从食用动物中分离沙门氏菌，并对分离株的抗菌药物耐药性进行表征。
对屠宰动物进行随机抽样。
肯尼亚内罗毕大学公共卫生、药理学与毒理学系以及德国新施塔特-玛丽恩塞的动物育种研究所。
分析了285份样本，包括粪便样本以及胴体、泄殖腔和咽拭子样本。
285份样本中有16份（5.6%）沙门氏菌呈阳性。猪胴体上沙门氏菌的流行率（19%）高于粪便中的流行率（8.6%）。鉴定出三种肠炎沙门氏菌亚种血清型，即圣保罗沙门氏菌（S. Saintpaul）、布伦德鲁普沙门氏菌（S. Braenderup）和海德堡沙门氏菌（S. Heidelberg），其中圣保罗沙门氏菌是主要血清型。在所有分离株中，35.7%发现有抗菌药物耐药性。海德堡沙门氏菌分离株对所有测试的抗菌药物均敏感。在耐药沙门氏菌分离株中，7.1%发现有多重耐药性。仅在海德堡沙门氏菌中检测到质粒。氨苄西林耐药性基于bla(TEM)基因的表达，而氯霉素、链霉素和四环素耐药性分别由catAl、strA和tet(A)基因编码。
猪可能是抗菌药物耐药性沙门氏菌的储存宿主，猪肉在屠宰场的交叉污染可能是食品安全风险。我们建议改善屠宰场卫生状况，以尽量减少猪胴体的污染。
