Terova Genciana, Rimoldi Simona, Larghi Stefano, Bernardini Giovanni, Gornati Rosalba, Saroglia Marco
Department of Biotechnology and Molecular Sciences, University of Insubria, Via JH Dunant, 3, 21100 Varese, Italy.
Biochem Biophys Res Commun. 2007 Nov 23;363(3):591-6. doi: 10.1016/j.bbrc.2007.09.013. Epub 2007 Sep 17.
In this study the sea bass (Dicentrarchus labrax) pepsinogen C gene was isolated. The nucleotide sequences of all exons are presented. The organization of the gene is compatible with that of other aspartic proteinases. The predicted 388-residue amino acid (aa) sequence of sea bass pepsinogen C consists of a signal sequence of 16 amino acid residues, an activation peptide of 43 residues, and the mature pepsin of 329 residues containing the two characteristic active-site aspartic acids. We also analyzed fasting-induced changes in the expression of progastricsin mRNA, using real-time RT-PCR absolute quantification. Progastricsin mRNA copy number was downregulated under conditions of negative energy balance, such as starvation, and upregulated during positive energy balance, such as refeeding. These findings offer new information about the sea bass progastricsin gene and support a role of this gastric digestive enzyme in the regulation of food intake in sea bass.
在本研究中，分离出了海鲈（欧洲鲈）的胃蛋白酶原C基因。列出了所有外显子的核苷酸序列。该基因的结构与其他天冬氨酸蛋白酶的结构相符。预测的海鲈胃蛋白酶原C的388个氨基酸残基序列由16个氨基酸残基的信号序列、43个残基的激活肽和包含两个特征性活性位点天冬氨酸的329个残基的成熟胃蛋白酶组成。我们还使用实时逆转录聚合酶链反应绝对定量分析了空腹诱导的前胃蛋白酶原mRNA表达的变化。在前胃蛋白酶原mRNA拷贝数在负能量平衡条件下（如饥饿）下调，而在正能量平衡条件下（如再投喂）上调。这些发现提供了有关海鲈前胃蛋白酶原基因的新信息，并支持这种胃消化酶在海鲈食物摄入调节中的作用。
