Almeida Jorge R, Price David A, Papagno Laura, Arkoub Zaïna Aït, Sauce Delphine, Bornstein Ethan, Asher Tedi E, Samri Assia, Schnuriger Aurélie, Theodorou Ioannis, Costagliola Dominique, Rouzioux Christine, Agut Henri, Marcelin Anne-Geneviève, Douek Daniel, Autran Brigitte, Appay Victor
Cellular Immunology Laboratory, U543, Institut National de la Santé et de la Recherche Médicale, Avenir Group, 75013 Paris, France.
J Exp Med. 2007 Oct 1;204(10):2473-85. doi: 10.1084/jem.20070784. Epub 2007 Sep 24.
The key attributes of CD8+ T cell protective immunity in human immunodeficiency virus (HIV) infection remain unclear. We report that CD8+ T cell responses specific for Gag and, in particular, the immunodominant p24 epitope KK10 correlate with control of HIV-1 replication in human histocompatibility leukocyte antigen (HLA)-B27 patients. To understand further the nature of CD8+ T cell-mediated antiviral efficacy, we performed a comprehensive study of CD8+ T cells specific for the HLA-B27-restricted epitope KK10 in chronic HIV-1 infection based on the use of multiparametric flow cytometry together with molecular clonotypic analysis and viral sequencing. We show that B27-KK10-specific CD8+ T cells are characterized by polyfunctional capabilities, increased clonal turnover, and superior functional avidity. Such attributes are interlinked and constitute the basis for effective control of HIV-1 replication. These data on the features of effective CD8+ T cells in HIV infection may aid in the development of successful T cell vaccines.
在人类免疫缺陷病毒（HIV）感染中，CD8 + T细胞保护性免疫的关键属性仍不清楚。我们报告称，针对Gag的CD8 + T细胞反应，特别是免疫显性的p24表位KK10，与人类组织相容性白细胞抗原（HLA）-B27患者体内HIV-1复制的控制相关。为了进一步了解CD8 + T细胞介导的抗病毒功效的本质，我们基于多参数流式细胞术、分子克隆型分析和病毒测序，对慢性HIV-1感染中针对HLA-B27限制性表位KK10的CD8 + T细胞进行了全面研究。我们发现，B27-KK10特异性CD8 + T细胞具有多功能能力、增加的克隆更新率和卓越的功能亲和力等特征。这些属性相互关联，构成了有效控制HIV-1复制的基础。这些关于HIV感染中有效CD8 + T细胞特征的数据可能有助于成功开发T细胞疫苗。
