Krusinová E, Klementová M, Kopecký J, Wohl P, Kazdová L, Mlejnek P, Pravenec M, Hill M, Pelikánová T
Institute for Clinical and Experimental Medicine, Diabetes Centre, Vídenská 1958/9, 140 21 Prague, Czech Republic.
Eur J Endocrinol. 2007 Oct;157(4):443-9. doi: 10.1530/EJE-07-0034.
The potential insulin-sensitizing function of angiotensin II type 1 receptor blockade (ARB) with regard to selected adipokines is not fully explained so far. Our study aimed to explore the influence of acute hyperinsulinaemia and acutely induced ARB on resistin and adiponectin concentrations and expressions in healthy subjects.
Plasma adipokines were measured: 1) at 0, 30 and 240 min of hyperinsulinaemic (1 mU/kg per min) euglycaemic (5 mmol/l) clamp (HEC), and 2) during HEC after acute ARB (losartan 200 mg; AT-HEC) using the same protocol, in eight healthy subjects. Needle biopsy of abdominal s.c. fat was performed at 0, 30 and 240 min of both clamps to assess the adipokines' expressions.
Comparing the glucose disposals of HEC and AT-HEC, no difference in insulin sensitivity was found. Plasma resistin increased equally during HEC and AT-HEC (P < 0.05). The expression of resistin in s.c. fat increased during HEC (P < 0.05), while no significant changes in expression were observed during AT-HEC. Plasma levels of adiponectin did not change during both clamps. Adiponectin expression increased during HEC (P < 0.05), while it did not change during AT-HEC.
In healthy subjects, acute hyperinsulinaemia is associated with an increase in plasma resistin independently of ARB, while plasma adiponectin is not influenced by insulin or ARB. The expressions of both resistin and adiponectin in s.c. adipose tissue are stimulated by acute hyperinsulinaemia, whereas losartan attenuates their insulin-stimulated expressions. This suggests a potential effect of losartanon adipokines' expression.
血管紧张素II 1型受体阻断剂（ARB）对特定脂肪因子的潜在胰岛素增敏作用目前尚未完全阐明。我们的研究旨在探讨急性高胰岛素血症和急性诱导的ARB对健康受试者抵抗素和脂联素浓度及表达的影响。
在8名健康受试者中进行如下检测：1）在高胰岛素正常血糖钳夹（HEC，胰岛素输注速率为1 mU/kg每分钟，血糖维持在5 mmol/l）的0、30和240分钟时测量血浆脂肪因子；2）在急性ARB（氯沙坦200 mg；AT-HEC）后按照相同方案在HEC期间测量血浆脂肪因子。在两种钳夹状态的0、30和240分钟时进行腹部皮下脂肪的针刺活检，以评估脂肪因子的表达。
比较HEC和AT-HEC的葡萄糖处置情况，未发现胰岛素敏感性有差异。在HEC和AT-HEC期间血浆抵抗素均同等程度增加（P < 0.05）。皮下脂肪中抵抗素的表达在HEC期间增加（P < 0.05），而在AT-HEC期间未观察到表达有显著变化。在两种钳夹状态下血浆脂联素水平均未改变。脂联素的表达在HEC期间增加（P < 0.05），而在AT-HEC期间未改变。
在健康受试者中，急性高胰岛素血症与血浆抵抗素增加相关，且独立于ARB，而血浆脂联素不受胰岛素或ARB影响。急性高胰岛素血症刺激皮下脂肪组织中抵抗素和脂联素的表达，而氯沙坦减弱它们受胰岛素刺激的表达。这提示氯沙坦对脂肪因子表达有潜在作用。
