健康瘦素年轻男性体内高胰岛素血症和高血糖对瘦素、脂联素及酰化刺激蛋白的调节作用

Regulation of leptin, adiponectin and acylation-stimulating protein by hyperinsulinaemia and hyperglycaemia in vivo in healthy lean young men.

作者信息

Faraj M, Beauregard G, Tardif A, Loizon E, Godbout A, Cianflone K, Vidal H, Rabasa-Lhoret R

机构信息

Unité métabolique, département de nutrition, faculté de médecine, université de Montréal, 2405, chemin Côte-Ste-Catherine, Montréal, Québec, H3T 1A8, Canada.

出版信息

Diabetes Metab. 2008 Sep;34(4 Pt 1):334-42. doi: 10.1016/j.diabet.2008.01.014. Epub 2008 Jun 17.

DOI:10.1016/j.diabet.2008.01.014

PMID:18562232

Abstract

AIM

Both type 1 and 2 diabetes are associated with differential regulation of leptin, adiponectin and ASP. Our aim was to examine whether or not acute hyperinsulinaemia and/or hyperglycaemia per se have differential regulation of these hormones in healthy subjects.

METHODS

We examined changes in leptin, adiponectin and ASP concentrations and subcutaneous white adipose tissue mRNA expression with 3-hour hyperinsulinaemic (HI, n=10), hyperglycaemic (HG, n=7) and hyperinsulinaemic-hyperglycaemic (HGHI, n=8) clamps in healthy lean young men. As somatostatin was used for the HG and HGHI clamps, a control somatostatin clamp was carried out (n=4). Changes in the expression of HKII and p85alpha Pi3K were examined as positive controls for the induction of gene expression by the insulin pathway.

RESULTS

HI, HG and HGHI clamps increased expression of HKII and p85alpha Pi3K while somatostatin did not. The HI clamp decreased serum adiponectin (-15%, P<0.001) and increased serum leptin (+11%, P=0.031), while the HG clamp reduced serum leptin (-20%, P=0.003). The HGHI clamp increased serum ASP (+21%, P=0.047) and expression of C3 (+26%, P=0.018) and leptin (+50%, P=0.024). Interestingly, the control somatostatin clamp suppressed both serum leptin (-17%, P=0.043) and adiponectin (-7%, P=0.020).

CONCLUSION

HG and/or HI per se regulated the concentrations and expression of leptin, adiponectin and ASP in healthy lean young men, suggesting a contribution to dysregulation of these hormones in diabetes.

摘要

目的

1型和2型糖尿病均与瘦素、脂联素和ASP的差异调节有关。我们的目的是研究急性高胰岛素血症和/或高血糖本身在健康受试者中对这些激素是否有差异调节作用。

方法

我们在健康的瘦年轻男性中，通过3小时的高胰岛素血症（HI，n = 10）、高血糖（HG，n = 7）和高胰岛素血症-高血糖（HGHI，n = 8）钳夹试验，检测了瘦素、脂联素和ASP浓度以及皮下白色脂肪组织mRNA表达的变化。由于HG和HGHI钳夹试验使用了生长抑素，因此进行了对照生长抑素钳夹试验（n = 4）。检测HKII和p85α Pi3K的表达变化，作为胰岛素途径诱导基因表达的阳性对照。

结果

HI、HG和HGHI钳夹试验增加了HKII和p85α Pi3K的表达，而生长抑素则没有。HI钳夹试验降低了血清脂联素（-15%，P < 0.001）并增加了血清瘦素（+11%，P = 0.031），而HG钳夹试验降低了血清瘦素（-20%，P = 0.003）。HGHI钳夹试验增加了血清ASP（+21%，P = 0.047）以及C3的表达（+26%，P = 0.018）和瘦素（+50%，P = 0.024）。有趣的是，对照生长抑素钳夹试验同时抑制了血清瘦素（-17%，P = 0.043）和脂联素（-7%，P = 0.020）。