Pentney R J, Quackenbush L J
Department of Anatomical Sciences, State University of New York, Buffalo 14214.
Alcohol Clin Exp Res. 1991 Dec;15(6):1024-30. doi: 10.1111/j.1530-0277.1991.tb05205.x.
Twelve-month-old Fischer 344 rats were fed a liquid diet containing 35% ethanol until they were 18 or 24 months old. Pair-fed and chow-fed control rats were matched to each ethanol-fed rat for concurrent treatment. Cerebellar Purkinje cell networks were measured in half of the rats at the end of the ethanol treatment and in the remaining rats after a subsequent 2-month recovery period. Chronic ethanol consumption resulted in significant elongation of terminal segments in the networks, and the unpaired terminal segments were the predominant sites of this growth. An increase in the duration of ethanol consumption from 24 to 48 weeks caused significantly greater segment elongation in the ethanol-fed rats in spite of the fact that circulating blood levels of ethanol declined markedly with the increased duration of treatment. During the same period of time, a pattern of terminal segment regression followed by terminal segment regrowth characterized age-induced changes in these networks. Thus the effects of long-term ethanol consumption were distinct from effects of concurrent aging processes in the Purkinje cell networks. There were significant interactions between the diets and the longer duration of treatment, such that as segments elongated in the ethanol-fed rats, they shortened in the pair-fed rats, and between the diets and the recovery period, such that as segments elongated during recovery in the pair-fed rats, they shortened in the ethanol-fed rats.(ABSTRACT TRUNCATED AT 250 WORDS)
给12个月大的Fischer 344大鼠喂食含35%乙醇的流质饮食，直至它们18或24个月大。将配对喂食和正常饮食的对照大鼠与每只乙醇喂养的大鼠进行匹配，以便同时进行处理。在乙醇处理结束时，对一半大鼠的小脑浦肯野细胞网络进行测量，对其余大鼠在随后2个月的恢复期后进行测量。长期摄入乙醇导致网络中终末节段显著延长，且未配对的终末节段是这种生长的主要部位。尽管随着处理时间延长，乙醇的循环血液水平显著下降，但乙醇喂养大鼠的乙醇摄入时间从24周增加到48周，仍导致节段延长显著增加。在同一时期，这些网络中年龄诱导的变化特征是终末节段先退化后再生长。因此，长期摄入乙醇的影响与浦肯野细胞网络中同时发生的衰老过程的影响不同。饮食与较长处理时间之间存在显著相互作用，即随着乙醇喂养大鼠的节段延长，配对喂养大鼠的节段缩短；饮食与恢复期之间也存在显著相互作用，即随着配对喂养大鼠在恢复期间节段延长，乙醇喂养大鼠的节段缩短。（摘要截选至250字）
