Molloy Eleanor J, O'Neill Amanda J, Grantham-Sloan Julie J, Sheridan-Pereira Margaret, Fitzpatrick John M, Webb David W, Watson R William G
Department of Surgery, Mater Misericordiae University Hospital, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Ireland.
Am J Perinatol. 2007 Oct;24(9):525-30. doi: 10.1055/s-2007-986678. Epub 2007 Sep 24.
Systemic hypoxia-ischemia at birth may alter the neonatal neutrophil phenotype. In this study, we evaluated alterations in perinatal neutrophil phenotype following systemic hypoxia-ischemia compared with normal controls. Neutrophils from adults (n = 15), normal newborns (n = 20), newborns requiring resuscitation at birth (n = 17), and their respective maternal samples were incubated alone or with lipopolysaccharide (LPS). Surface receptor CD11b (neutrophil activation) and the percentage apoptosis (persistence of inflammatory response) were assessed using flow cytometry. Neutrophil apoptosis was decreased in neonates requiring resuscitation at birth and was further exaggerated in infants who developed mild neurological signs. All infants who required resuscitation were LPS hyporesponsive irrespective of neurological findings. Newborns with severe neurological signs had increased apoptosis and decreased CD11b. Maternal neutrophils were LPS hyporesponsive only if their infants had moderate/severe neurological signs. Infants with mild encephalopathy may display a predominantly proinflammatory neutrophil response with a persistent inflammatory response, whereas those with moderate/severe encephalopathy have a tendency toward immunosuppression.
出生时的全身性缺氧缺血可能会改变新生儿中性粒细胞的表型。在本研究中，我们评估了与正常对照组相比，全身性缺氧缺血后围产期中性粒细胞表型的变化。将来自成人（n = 15）、正常新生儿（n = 20）、出生时需要复苏的新生儿（n = 17）及其各自母亲的样本中的中性粒细胞单独或与脂多糖（LPS）一起孵育。使用流式细胞术评估表面受体CD11b（中性粒细胞活化）和凋亡百分比（炎症反应的持续性）。出生时需要复苏的新生儿中性粒细胞凋亡减少，而出现轻度神经学体征的婴儿中这种情况进一步加重。所有需要复苏的婴儿无论神经学检查结果如何均对LPS反应低下。有严重神经学体征的新生儿凋亡增加而CD11b减少。只有当母亲的婴儿有中度/重度神经学体征时，母亲的中性粒细胞才对LPS反应低下。患有轻度脑病的婴儿可能表现出以促炎为主的中性粒细胞反应和持续的炎症反应，而患有中度/重度脑病的婴儿则有免疫抑制倾向。
