• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

将DNA共价固定到聚苯乙烯微孔板上：分子仅在5'端结合。

Covalent immobilization of DNA onto polystyrene microwells: the molecules are only bound at the 5' end.

作者信息

Rasmussen S R, Larsen M R, Rasmussen S E

机构信息

A/S Nunc, Roskilde, Denmark.

出版信息

Anal Biochem. 1991 Oct;198(1):138-42. doi: 10.1016/0003-2697(91)90518-x.

DOI:10.1016/0003-2697(91)90518-x
PMID:1789415
Abstract

Carbodiimide-mediated condensation of DNA onto microwells is investigated. DNA is bound onto the microwells by formation of a phosphoramidate bond between the 5' terminal phosphate group and the microwells. Immobilization of 25 to 30 ng DNA per well is obtained. DNA molecules bound covalently at only the 5' end are, ideally, perfect for hybridization. The practicability of DNA molecules bound to microwells for hybridization is investigated.

摘要

研究了碳二亚胺介导的DNA在微孔上的缩合。通过5'末端磷酸基团与微孔之间形成磷酰胺键，将DNA结合到微孔上。每孔可固定25至30 ng DNA。理想情况下，仅在5'端共价结合的DNA分子非常适合杂交。研究了结合到微孔上的DNA分子用于杂交的实用性。

相似文献

1
Covalent immobilization of DNA onto polystyrene microwells: the molecules are only bound at the 5' end.将DNA共价固定到聚苯乙烯微孔板上：分子仅在5'端结合。
Anal Biochem. 1991 Oct;198(1):138-42. doi: 10.1016/0003-2697(91)90518-x.
2
Amination of polystyrene microwells: application to the covalent grafting of DNA probes for hybridization assays.聚苯乙烯微孔板的胺化：用于杂交分析的DNA探针共价接枝的应用。
Anal Biochem. 1996 Apr 5;236(1):85-94. doi: 10.1006/abio.1996.0135.
3
Covalent immobilization of biomolecules onto polystyrene MicroWells for use in biospecific assays.用于生物特异性分析的生物分子在聚苯乙烯微孔板上的共价固定。
Ann Biol Clin (Paris). 1990;48(9):647-50.
4
The use of 96-well polystyrene plates for DNA hybridization-based assays: an evaluation of different approaches to oligonucleotide immobilization.
Anal Biochem. 1995 May 1;227(1):201-9. doi: 10.1006/abio.1995.1271.
5
Comparison between microwell and bead supports for the detection of human cytomegalovirus amplicons by sandwich hybridization.用于通过夹心杂交检测人巨细胞病毒扩增子的微孔板和磁珠载体之间的比较。
Anal Biochem. 1997 Nov 15;253(2):180-9. doi: 10.1006/abio.1997.2352.
6
Controlled assembly of mesoscale structures using DNA as molecular bridges.利用DNA作为分子桥实现中尺度结构的可控组装。
J Am Chem Soc. 2002 Jul 24;124(29):8508-9. doi: 10.1021/ja017653f.
7
Optimizing the immobilization of single-stranded DNA onto glass beads.优化单链DNA在玻璃珠上的固定化。
J Biochem Biophys Methods. 2001 Feb 26;47(3):221-31. doi: 10.1016/s0165-022x(00)00146-9.
8
The use of RNA probes for the analysis of gene expression. Northern blot hybridization and ribonuclease protection assay.用于基因表达分析的RNA探针。Northern印迹杂交和核糖核酸酶保护分析。
Methods Mol Biol. 1998;86:87-102. doi: 10.1385/0-89603-494-1:87.
9
PCR product quantification by non-radioactive hybridization procedures using an oligonucleotide covalently bound to microwells.
Mol Cell Probes. 1993 Jun;7(3):187-97. doi: 10.1006/mcpr.1993.1028.
10
Use of asymmetric PCR to generate long primers and single-stranded DNA for incorporating cross-linking analogs into specific sites in a DNA probe.使用不对称PCR生成用于将交联类似物掺入DNA探针特定位点的长引物和单链DNA。
Genome Res. 1996 Sep;6(9):886-92. doi: 10.1101/gr.6.9.886.

引用本文的文献

1
Scanometric Detection of Tomato Leaf Curl New Delhi Viral DNA Using Mono- and Bifunctional AuNP-Conjugated Oligonucleotide Probes.使用单功能和双功能金纳米颗粒偶联寡核苷酸探针扫描检测番茄卷叶新德里病毒DNA
ACS Omega. 2019 Jun 11;4(6):10094-10107. doi: 10.1021/acsomega.9b00340. eCollection 2019 Jun 30.
2
Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates.用于合成基于DNA的生物共轭物的共价交联策略的表征
J Biol Eng. 2019 Jul 10;13:63. doi: 10.1186/s13036-019-0191-2. eCollection 2019.
3
Electrochemical DNA hybridization sensors based on conducting polymers.
基于导电聚合物的电化学DNA杂交传感器。
Sensors (Basel). 2015 Feb 5;15(2):3801-29. doi: 10.3390/s150203801.
4
Gold nanoparticles stabilized with MPEG-grafted poly(l-lysine): in vitro and in vivo evaluation of a potential theranostic agent.用聚乙二醇单甲醚接枝聚（L-赖氨酸）稳定的金纳米颗粒：一种潜在的诊疗剂的体外和体内评价
Bioconjug Chem. 2015 Jan 21;26(1):39-50. doi: 10.1021/bc5005087. Epub 2014 Dec 11.
5
Cell penetrable humanized-VH/V(H)H that inhibit RNA dependent RNA polymerase (NS5B) of HCV.可穿透细胞的人源化 VH/V(H)H，抑制丙型肝炎病毒的 RNA 依赖的 RNA 聚合酶（NS5B）。
PLoS One. 2012;7(11):e49254. doi: 10.1371/journal.pone.0049254. Epub 2012 Nov 8.
6
DNA sensors with diamond as a promising alternative transducer material.以钻石为有前途的替代换能材料的 DNA 传感器。
Sensors (Basel). 2009;9(7):5600-36. doi: 10.3390/s90705600. Epub 2009 Jul 14.
7
Facile DNA immobilization on surfaces through a catecholamine polymer.通过儿茶酚胺聚合物将DNA轻松固定在表面。
Angew Chem Int Ed Engl. 2011 Jan 17;50(3):732-6. doi: 10.1002/anie.201005001. Epub 2010 Dec 22.
8
In situ oligonucleotide synthesis on carbon materials: stable substrates for microarray fabrication.碳材料上的原位寡核苷酸合成：用于微阵列制造的稳定基质。
Nucleic Acids Res. 2008 Jan;36(1):e7. doi: 10.1093/nar/gkm1103. Epub 2007 Dec 15.
9
Diamond and biology.钻石与生物学。
J R Soc Interface. 2007 Jun 22;4(14):439-61. doi: 10.1098/rsif.2006.0196.
10
Development of a PCR assay followed by nonradioactive hybridization using oligonucleotides covalently bound to CovaLink NH microwells for detection of four Plasmodium species in blood samples from humans.开发一种聚合酶链反应（PCR）检测方法，随后使用与CovaLink NH微孔共价结合的寡核苷酸进行非放射性杂交，以检测人类血液样本中的四种疟原虫。
J Clin Microbiol. 2006 Sep;44(9):3279-84. doi: 10.1128/JCM.00014-06.