将DNA共价固定到聚苯乙烯微孔板上：分子仅在5'端结合。
Covalent immobilization of DNA onto polystyrene microwells: the molecules are only bound at the 5' end.
作者信息
Rasmussen S R, Larsen M R, Rasmussen S E
机构信息
A/S Nunc, Roskilde, Denmark.
出版信息
Anal Biochem. 1991 Oct;198(1):138-42. doi: 10.1016/0003-2697(91)90518-x.
PMID:1789415
Abstract
Carbodiimide-mediated condensation of DNA onto microwells is investigated. DNA is bound onto the microwells by formation of a phosphoramidate bond between the 5' terminal phosphate group and the microwells. Immobilization of 25 to 30 ng DNA per well is obtained. DNA molecules bound covalently at only the 5' end are, ideally, perfect for hybridization. The practicability of DNA molecules bound to microwells for hybridization is investigated.
摘要
研究了碳二亚胺介导的DNA在微孔上的缩合。通过5'末端磷酸基团与微孔之间形成磷酰胺键，将DNA结合到微孔上。每孔可固定25至30 ng DNA。理想情况下，仅在5'端共价结合的DNA分子非常适合杂交。研究了结合到微孔上的DNA分子用于杂交的实用性。
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