Rasmussen S E
NUNC Laboratories, Ros-Kilde, Denmark.
Ann Biol Clin (Paris). 1990;48(9):647-50.
Modification of polystyrene for higher binding capacity and/or for specific covalent immobilization of biomolecules is discussed. The benefit of covalent coupling of biomolecules onto a new commercially available surface type for covalent immobilization, CovaLink NH, is illustrated. The CovaLink NH solid phase has spacer arms covalently grafted onto the polystyrene solid phase, approximately 10(14) groups/cm2. Coupling procedures for covalent immobilization of biotin and peptides are demonstrated, and the advantage of using carbodiimide for coupling of carboxylic acid containing compounds is shown.
讨论了对聚苯乙烯进行改性以提高结合能力和/或实现生物分子的特异性共价固定。阐述了将生物分子共价偶联到一种新型市售用于共价固定的表面类型CovaLink NH上的益处。CovaLink NH固相具有共价接枝到聚苯乙烯固相上的间隔臂，约为10(14)个基团/平方厘米。展示了生物素和肽共价固定的偶联程序，并显示了使用碳二亚胺偶联含羧酸化合物的优势。
