A membrane blotting method for following the time course of protein radioiodination using Iodobeads.
作者信息
Cheng H, Rudick M J
机构信息
Biology Department, Texas Woman's University, Denton 76204-1971.
出版信息
Anal Biochem. 1991 Oct;198(1):191-3. doi: 10.1016/0003-2697(91)90527-z.
PMID:1789424
Abstract
A method for monitoring and optimizing the incorporation of radioiodide into protein is described. The use of immobilized chloramine-T (Iodobeads) combined with timed blots on nitrocellulose forms the basis of an efficient assay system. Results with Iodobeads and with the commonly utilized soluble form of chloramine-T are compared.
摘要
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