Woltanski K P, Besch W, Keilacker H, Ziegler M, Kohnert K D
Central Institute of Diabetes, Gerhardt Katsch, Karlsburg/GDR.
Exp Clin Endocrinol. 1990 Feb;95(1):39-46. doi: 10.1055/s-0029-1210932.
Following optimization of the reaction conditions, e.g. concentration of oxidizing agents, reaction time, volume of reaction mixture, and pH, chloramine T and the new iodination reagent, Iodogen, were compared for their effectiveness in radioiodination of insulin, glucagon, human growth hormone (hGH), and rabbit anti-mouse IgG. The radioactive peptide hormones prepared were analyzed for the presence of aggregate and breakdown products by polyacrylamide gel electrophoresis (PAGE) at pH 8.9, the rabbit anti-mouse IgG was tested for the presence of low molecular weight damage products by gel filtration on Sephadex G-50. The results demonstrate that with respect to iodine incorporation, specific activity, and immunological reactivity either method can be used to prepare under carefully controlled conditions a wide range of tracers with high specific activity at minimal oxidation damage. These tracers are shown to be highly suitable in radioimmunoassays after previous purification by PAGE and gel filtration, respectively.
在优化反应条件（如氧化剂浓度、反应时间、反应混合物体积和pH值）之后，比较了氯胺T和新型碘化试剂碘代甘氨酸在胰岛素、胰高血糖素、人生长激素（hGH）和兔抗小鼠IgG放射性碘化反应中的有效性。通过在pH 8.9条件下的聚丙烯酰胺凝胶电泳（PAGE）分析制备的放射性肽激素中聚集物和分解产物的存在情况，通过在Sephadex G - 50上进行凝胶过滤测试兔抗小鼠IgG中低分子量损伤产物的存在情况。结果表明，就碘掺入、比活性和免疫反应性而言，在精心控制的条件下，两种方法均可用于制备一系列具有高比活性且氧化损伤最小的示踪剂。经PAGE和凝胶过滤分别纯化后，这些示踪剂在放射免疫分析中显示出高度适用性。
