液体闪烁计数法同时测定³H、¹⁴C和²²Na在细胞离子转运测量中的应用。

Application of simultaneous determination of 3H, 14C, and 22Na by liquid scintillation counting to the measurement of cellular ion-transport.

作者信息

Miyazawa Y, Sakai N, Murakami N, Konishi T

机构信息

Department of Radiochemistry--Biophysics, Niigata College of Pharmacy, Japan.

出版信息

Anal Biochem. 1991 Oct;198(1):194-9. doi: 10.1016/0003-2697(91)90528-2.

DOI:10.1016/0003-2697(91)90528-2

PMID:1789425

Abstract

A liquid scintillation counting method for simultaneous determination of three radioactive nuclides (3H, 14C, and 22Na) of biological interest was studied. By comparing the beta spectra of the three nuclides, their counting energy ranges, A, B, and C, were determined. 22NA was set high enough to avoid any spillover counts from lower-energy nuclides. Region A for 3H was set to maximize the counting efficiency. A good correlation between the counting efficiency for 22Na in region C and the counting efficiency of other nuclides in all regions was obtained. Prior to 3H and 14C dpm calculations, the 22Na counts spilled down in regions A and B were subtracted from the total counts in regions A and B. A simple linear equation was then used to compute 3H and 14C dpm. Findings show that the method presented is adaptable for highly quenched samples up to quenching indices of tSIE = 100. The method is useful for studying the biological transport coupled to Na+.

摘要

研究了一种用于同时测定三种具有生物学意义的放射性核素（³H、¹⁴C 和 ²²Na）的液体闪烁计数法。通过比较这三种核素的β能谱，确定了它们的计数能量范围 A、B 和 C。²²Na 的设置足够高，以避免来自低能核素的任何溢出计数。³H 的区域 A 设置为使计数效率最大化。在区域 C 中²²Na 的计数效率与所有区域中其他核素的计数效率之间获得了良好的相关性。在计算³H 和¹⁴C 的每分钟衰变数（dpm）之前，从区域 A 和 B 的总计数中减去在区域 A 和 B 中向下溢出的²²Na 计数。然后使用一个简单的线性方程来计算³H 和¹⁴C 的 dpm。研究结果表明，所提出的方法适用于淬灭指数高达 tSIE = 100 的高度淬灭样品。该方法对于研究与 Na⁺ 相关的生物转运很有用。