在通过液体闪烁法测定³H、¹⁴C和³⁵S之前对植物组织进行脱色和增溶处理。
Decoloration and solubilization of plant tissue prior to determination of 3H, 14C, and 35S by liquid scintillation.
作者信息
Smith I K, Lang A L
机构信息
Department of Botany, Ohio University, Athens 45701.
出版信息
Anal Biochem. 1987 Aug 1;164(2):531-6. doi: 10.1016/0003-2697(87)90529-x.
PMID:3674398
Abstract
A method is described for the decoloration and partial solubilization of plant tissue with 2% sodium hypochlorite. Following treatment of the digest with ammonia, the samples are suitable for the determination of 3H, 14C, and 35S by liquid scintillation counting. The color quenching is negligible and counting efficiencies are high: 30-40% for 3H and 90-95% for 14C.
摘要
描述了一种用2%次氯酸钠对植物组织进行脱色和部分溶解的方法。用氨水处理消化物后，样品适用于通过液体闪烁计数法测定³H、¹⁴C和³⁵S。颜色淬灭可忽略不计，计数效率很高：³H为30 - 40%，¹⁴C为90 - 95%。
Zotero 插件