Madueño F, Guerrero M G
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Spain.
Anal Biochem. 1991 Oct;198(1):200-2. doi: 10.1016/0003-2697(91)90529-3.
A sensitive procedure for the determination of nitrate within the 1- to 50-microM range is described. The method is based on the photoreduction of nitrate to nitrite by whole cells of a nitrite reductase-less mutant strain of the unicellular cyanobacterium Synechococcus R2. Cell suspensions of this cyanobacterium retain high levels of nitrate photoreduction activity for at least 2 months when maintained in the presence of dimethyl sulfoxide at -70 degrees C.
本文描述了一种用于测定1至50微摩尔范围内硝酸盐的灵敏方法。该方法基于单细胞蓝藻聚球藻R2亚硝酸还原酶缺陷突变株的全细胞将硝酸盐光还原为亚硝酸盐。当在-70℃下于二甲基亚砜存在的情况下保存时，这种蓝藻的细胞悬液可保持高水平的硝酸盐光还原活性至少2个月。
