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海洋固氮丝状蓝藻束毛藻属(Trichodesmium sp.)菌株WH9601的硝酸盐同化基因。

Nitrate assimilation genes of the marine diazotrophic, filamentous cyanobacterium Trichodesmium sp. strain WH9601.

作者信息

Wang Q, Li H, Post A F

机构信息

"H. Steinitz" Marine Biology Laboratory, Hebrew University, Coral Beach, 88103 Eilat, Israel.

出版信息

J Bacteriol. 2000 Mar;182(6):1764-7. doi: 10.1128/JB.182.6.1764-1767.2000.

Abstract

A 4.0-kb DNA fragment of Trichodesmium sp. strain WH9601 contained gene sequences encoding the nitrate reduction enzymes, nirA and narB. A third gene positioned between nirA and narB encodes a putative membrane protein with similarity to the nitrate permeases of Bacillus subtilis (NasA) and Emericella nidulans (CrnA). The gene was shown to functionally complement a DeltanasA mutant of B. subtilis and was assigned the name napA (nitrate permease). NapA was involved in both nitrate and nitrite uptake by the complemented B. subtilis cells. napA is distinct from the nrt genes that encode the nitrate transporter of freshwater cyanobacteria.

摘要

束毛藻属菌株WH9601的一个4.0千碱基DNA片段包含编码硝酸还原酶nirA和narB的基因序列。位于nirA和narB之间的第三个基因编码一种假定的膜蛋白,该蛋白与枯草芽孢杆菌(NasA)和构巢曲霉(CrnA)的硝酸盐通透酶相似。该基因在功能上可互补枯草芽孢杆菌的ΔnasA突变体,并被命名为napA(硝酸盐通透酶)。NapA参与了互补后的枯草芽孢杆菌细胞对硝酸盐和亚硝酸盐的摄取。napA与编码淡水蓝细菌硝酸盐转运蛋白的nrt基因不同。

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