Compagnon Isabelle, Dugourd Philippe
Université de Lyon, Université Lyon 1, CNRS, LASIM UMR 5579, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
J Phys Chem A. 2007 Oct 25;111(42):10635-9. doi: 10.1021/jp073309e. Epub 2007 Sep 26.
A variation of the kinetic method for the analysis of fragmentation patterns in mass spectra is proposed. The procedure presents three notable features: no evaluation of the effective temperature of the parent ion is required; the ratio of the activation energies for all competitive channels at play are provided; and the measurement is not biased by the mass discrimination of the instrument. The method is based on the analysis of mass spectra recorded as a function of both the excitation energy and the excitation time. Collision-activated dissociation of protonated Leu-enkephalin achieved in a quadrupolar ion trap and analyzed with this method is presented.
提出了一种用于分析质谱中碎裂模式的动力学方法的变体。该程序具有三个显著特点：无需评估母离子的有效温度；提供了所有竞争通道的活化能之比；测量不受仪器质量歧视的影响。该方法基于对作为激发能量和激发时间函数记录的质谱的分析。本文展示了在四极离子阱中实现的质子化亮氨酸脑啡肽的碰撞活化解离，并使用该方法进行了分析。
