Vidal Martin A, Kilroy Gail E, Lopez Mandi J, Johnson Jill R, Moore Rustin M, Gimble Jeffrey M
Equine Health Studies Program, Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Vet Surg. 2007 Oct;36(7):613-22. doi: 10.1111/j.1532-950X.2007.00313.x.
To characterize equine adipose tissue-derived stromal cell (ASC) frequency and growth characteristics and assess of their adipogenic and osteogenic differentiation potential.
In vitro experimental study.
Horses (n=5; aged, 9 months to 5 years).
Cell doubling characteristics of ASCs harvested from supragluteal subcutaneous adipose tissue were evaluated over 10 passages. Primary, second (P2), and fourth (P4) passage ASCs were induced under appropriate conditions to undergo adipogenesis and osteogenesis. Limit dilution assays were performed on each passage to determine the frequency of colony-forming units with a fibroblastic (CFU-F) phenotype and the frequency of ASC differentiation into the adipocyte (CFU-Ad) and osteoblast (CFU-Ob) phenotype.
ASC isolates exhibited an average cell-doubling time of 2.1+/-0.9 days during the first 10 cell doublings. Approximately 1 in 2.3+/-0.4 of the total stromal vascular fraction nucleated cells were ASCs, based on the CFU-F assays, and 1 in 3.6+/-1.3 expressed alkaline phosphatase, an osteogenic marker. Primary ASCs differentiated in response to adipogenic (1 in 4.9+/-5.4, CFU-Ad) and osteogenic (1 in <2.44, CFU-Ob) inductive conditions and maintained their differentiation potential during subsequent passages (P2 and P4).
The frequency, in vitro growth rate, and adipogenic and osteogenic differentiation potential of equine ASCs show some differences to those documented for ASCs in other mammalian species.
Adipose tissue is a potential source of adult stem cells for tissue engineering applications in equine veterinary medicine.
鉴定马脂肪组织来源的基质细胞（ASC）的频率和生长特性，并评估其成脂和成骨分化潜能。
体外实验研究。
马（n = 5；年龄9个月至5岁）。
对从臀上皮下脂肪组织采集的ASC进行10代传代培养，评估其细胞倍增特性。在适当条件下诱导原代、第二代（P2）和第四代（P4）传代的ASC进行成脂和成骨分化。对每一代进行极限稀释分析，以确定具有成纤维细胞（CFU-F）表型的集落形成单位频率以及ASC分化为脂肪细胞（CFU-Ad）和成骨细胞（CFU-Ob）表型的频率。
在最初的10次细胞倍增过程中，ASC分离株的平均细胞倍增时间为2.1±0.9天。基于CFU-F分析，总的基质血管成分有核细胞中约2.3±0.4个中有1个是ASC，并且3.6±1.3个中有1个表达碱性磷酸酶，这是一种成骨标志物。原代ASC在成脂（4.9±5.4个中有1个，CFU-Ad）和成骨（<2.44个中有1个，CFU-Ob）诱导条件下发生分化，并在随后的传代（P2和P4）中保持其分化潜能。
马ASC的频率、体外生长速率以及成脂和成骨分化潜能与其他哺乳动物物种中记录的ASC存在一些差异。
脂肪组织是马兽医医学中用于组织工程应用的成体干细胞的潜在来源。
