Orzoł Dominika, Kępska Martyna, Zyzak Magdalena
Department of Experimental Biology, Institute of Biology, Wrocław University of Environmental and Life Sciences, C. K. Norwida 25, 50-375 Wrocław, Poland.
Int J Mol Sci. 2025 Jul 17;26(14):6867. doi: 10.3390/ijms26146867.
Equine metabolic syndrome (EMS) is a prevalent endocrine disorder associated with insulin dysregulation, oxidative stress, and impaired regenerative capacity of adipose-derived stem cells (ASCs). The aim of this study was to evaluate the effects of orientin-a plant-derived flavonoid with known antioxidant properties-on equine ASCs (EqASCs) derived from both clinically healthy and diagnosed EMS-affected mares. EqASCs were treated with orientin to evaluate its biological effects. The analysis included key cellular functions such as proliferative capacity, viability, apoptosis, oxidative stress, senescence, clonogenicity, and migration. Orientin significantly enhanced the proliferative activity of EqASCs, as evidenced by increased Ki67 expression and favorable alterations in cell cycle distribution. In addition, the treatment improved overall cell viability, reduced apoptotic activity, and restored both the clonogenic potential and migratory capacity of the cells, with particularly pronounced effects observed in EqASCs isolated from EMS-affected horses. Importantly, orientin also led to a marked reduction in cellular senescence and oxidative stress, further suggesting its potential as a protective and regenerative agent in metabolically impaired ASCs. These findings indicate that orientin can exert comprehensive cytoprotective effects on EqASCs, with pronounced benefits in cells derived from EMS-affected animals. By improving multiple functional parameters, orientin emerges as a promising candidate for therapeutic strategies aimed at restoring the regenerative potential of ASCs compromised by metabolic dysregulation in horses.
马代谢综合征（EMS）是一种常见的内分泌疾病，与胰岛素调节异常、氧化应激以及脂肪来源干细胞（ASC）的再生能力受损有关。本研究的目的是评估荭草素（一种具有已知抗氧化特性的植物来源黄酮类化合物）对临床健康和已诊断为受EMS影响的母马所来源的马ASC（EqASC）的作用。用荭草素处理EqASC以评估其生物学效应。分析包括关键的细胞功能，如增殖能力、活力、凋亡、氧化应激、衰老、克隆形成能力和迁移。荭草素显著增强了EqASC的增殖活性，Ki67表达增加以及细胞周期分布的有利改变证明了这一点。此外，该处理提高了总体细胞活力，降低了凋亡活性，并恢复了细胞的克隆形成潜力和迁移能力，在从受EMS影响的马分离的EqASC中观察到特别明显的效果。重要的是，荭草素还导致细胞衰老和氧化应激显著降低，进一步表明其作为代谢受损ASC中的保护和再生剂的潜力。这些发现表明荭草素可以对EqASC发挥全面的细胞保护作用，对来自受EMS影响动物的细胞有明显益处。通过改善多个功能参数，荭草素成为旨在恢复因马代谢失调而受损的ASC再生潜力的治疗策略的有希望的候选者。
