Joosten I, Sanders M F, Hensen E J
Department of Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Anim Genet. 1991;22(6):455-63. doi: 10.1111/j.1365-2052.1991.tb00717.x.
The possibility was examined that in cattle compatibility of major histocompatibility complex (MHC) products between dam and calf might negatively influence the placental maturation and expulsion, and therefore increase the risk of retained placenta in healthy, normally calving cattle. Fifteen combinations of a single dam and two offspring were selected; the placenta of the first offspring was normally expelled (control) and the placenta of the second one was retained (case). The MHC class I and class II antigens of the animals were typed by immunoprecipitation and by one-dimensional isoelectric focusing (1D-IEF). Compatibility or incompatibility of class I or class II antigens was established by comparison of the IEF banding patterns of dam and calves. Analysis revealed that MHC class I compatibility between dam and calf increased the risk of retained placenta. In this study, the effect of class II compatibility was not significant, nor was the effect of the interaction of class I and class II. In a subsequent, additional sample the experimental design was extended: induction of tolerance against non-inherited maternal antigens (NIMA) might be implicated in the occurrence of the disorder within the group of class I incompatible cases. In three out of the five class I incompatible retained placenta cases, the banding pattern of the incompatible haplotype of the calf was identical to that of the haplotype of the granddam that was not inherited by the dam (NIMA). Notably, within the nine class I incompatible controls, there were none in which the offspring shared a paternal class I type with the granddam. This might suggest a tolerance-inducing effect of NIMA in cattle in relation to retained placenta.(ABSTRACT TRUNCATED AT 250 WORDS)
在母牛和小牛之间,主要组织相容性复合体(MHC)产物的相容性可能会对胎盘成熟和排出产生负面影响,从而增加健康、正常产犊母牛发生胎盘滞留的风险。选择了15组由一头母牛和两头后代组成的组合;第一头后代的胎盘正常排出(对照组),第二头后代的胎盘滞留(病例组)。通过免疫沉淀和一维等电聚焦(1D-IEF)对动物的MHC I类和II类抗原进行分型。通过比较母牛和小牛的IEF条带模式来确定I类或II类抗原的相容性或不相容性。分析表明,母牛和小牛之间的MHC I类相容性增加了胎盘滞留的风险。在本研究中,II类相容性的影响不显著,I类和II类相互作用的影响也不显著。在随后的另一个样本中,扩展了实验设计:对非遗传母体抗原(NIMA)的耐受性诱导可能与I类不相容病例组中该疾病的发生有关。在5例I类不相容的胎盘滞留病例中,有3例小牛不相容单倍型的条带模式与未被母牛遗传的祖母单倍型的条带模式相同(NIMA)。值得注意的是,在9例I类不相容的对照组中,没有一例后代与祖母共享父本I类类型。这可能表明NIMA在牛中对胎盘滞留具有耐受性诱导作用。(摘要截短至250字)