Ekström Jens-Ola, Tolf Conny, Edman Kjell-A, Lindberg A Michael
School of Pure and Applied Natural Sciences, University of Kalmar, Kalmar, Sweden.
Microbiol Immunol. 2007;51(9):841-50. doi: 10.1111/j.1348-0421.2007.tb03980.x.
It is of great importance to know how a virus particle is affected by environmental conditions. Physicochemical properties of the virion will affect the virus viability in different environments, viral transmission between hosts, and will also be important for safe handling of the virus. The physicochemical properties of the Ljungan virus (LV) prototype, 87-012, adapted to grow in cell culture were evaluated using both LV in crude cell extracts and purified virions. Replication of LV was completely inhibited by heat. Titers of LV were unaffected by acidic pH, reduced but not completely abolished by alkaline pH, and unaffected by exposure to the detergents Triton X-100 and SDS. Surprisingly, viable LV was still detected after incubation in the acidic, oxidising and detergent-containing environment produced by the commonly used disinfectant Virkon. In conclusion, LV is resilient to extreme pH, detergents and also to oxidising environments, but is sensitive to heat treatment.
了解病毒颗粒如何受到环境条件的影响至关重要。病毒粒子的物理化学性质会影响病毒在不同环境中的生存能力、宿主之间的病毒传播，对于病毒的安全处理也很重要。使用粗细胞提取物中的Ljungan病毒（LV）原型87 - 012和纯化的病毒粒子，对适应在细胞培养中生长的LV的物理化学性质进行了评估。LV的复制完全被热抑制。LV的滴度不受酸性pH的影响，在碱性pH下降低但未完全消除，并且不受去污剂Triton X - 100和SDS暴露的影响。令人惊讶的是，在常用消毒剂Virkon产生的酸性、氧化和含洗涤剂的环境中孵育后，仍检测到有活力的LV。总之，LV对极端pH、去污剂以及氧化环境具有抗性，但对热处理敏感。
