来自黄绿木霉DY-59和微小根毛霉VS-9的几丁质酶的抗真菌活性
Antifungal activity of chitinases from Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9.
作者信息
Nguyen Nam Van, Kim Young-Ju, Oh Kyung-Taek, Jung Woo-Jin, Park Ro-Dong
机构信息
Glucosamine Saccharide Materials, National Research Laboratory, Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 500-757, South Korea.
出版信息
Curr Microbiol. 2008 Jan;56(1):28-32. doi: 10.1007/s00284-007-9033-4.
Two chitinolytic fungal strains, Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9, were isolated from soil samples of Korea and Vietnam, respectively. DY-59 and VS-9 crude chitinases secreted by these fungi in the 0.5% swollen chitin culture medium had an optimal pH of 4 and the optimal temperatures of 40 degrees C and 60 degrees C, respectively. Enzymatic hydrolysis products from crab swollen chitin were N-acetyl-beta-D-glucosamine (GlcNAc) by DY-59 chitinase, and GlcNAc and N, N'-diacetylchitobiose (GlcNAc)2 by VS-9 chitinases. The chitinases degraded the cell wall of Fusarium solani hyphae to produce oligosaccharides, among which GlcNAc, (GlcNAc)2, and pentamer (GlcNAc)5 were identified by high-pressure liquid chromatography. DY-59 and VS-9 chitinases inhibited F. solani microconidial germination by more than 70% and 60% at final protein concentrations of 5 and 27 microg mL(-1), respectively, at 30 degrees C for 20 h treatment.
分别从韩国和越南的土壤样本中分离出两种具有几丁质分解能力的真菌菌株，即绿色木霉DY - 59和微小根霉VS - 9。这些真菌在0.5%溶胀几丁质培养基中分泌的DY - 59和VS - 9粗几丁质酶的最适pH值为4，最适温度分别为40℃和60℃。DY - 59几丁质酶作用于蟹溶胀几丁质的酶解产物为N - 乙酰 - β - D - 葡萄糖胺（GlcNAc），而VS - 9几丁质酶的酶解产物为GlcNAc和N，N'-二乙酰壳二糖（GlcNAc）2。几丁质酶降解了茄丝核菌菌丝的细胞壁以产生寡糖，其中通过高压液相色谱法鉴定出了GlcNAc、（GlcNAc）2和五聚体（GlcNAc）5。在30℃处理20小时时，DY - 59和VS - 9几丁质酶在终蛋白浓度分别为5和27μg mL（-1）时，对茄丝核菌微分生孢子萌发的抑制率分别超过70%和60%。
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