Woodard J F, Srinivasan S R, Zimny M L, Radhakrishnamurphy B, Berenson G S
Lab Invest. 1976 May;34(5):516-21.
Lipoprotein-glycosaminoglycan (GAG) complexes were isolated from fibrous plaque lesions of human aortas and examined by electron microscopy. After fractionation by gel chromatography and ultracentrifugation, both very low density lopoprotein-GAG and low density lopoprotein-GAG complexes showed particles which were mainly 1,000 to 2,000 A in diameter. Occasional large aggregations 3,000 to 10,000 A in diameter were seen after gel filtration and in the very low density lipoprotein fraction of complexes. In general, the complexes were larger than serum very low density lipoprotein and low density lipoprotein, although serum very low density lipoprotein particles ranged from 250 to 2,000 A. In vitro low density lipoprotein-heparin complexes consisted of spherical particles generally 1,000 to 2,000 A in diameter formed by the aggregation and coalescing of smaller low density liproprotein particles in the presence of heparin and Ca2+. These observations support a concept that GAG of the aortic intimal "ground substance" sequester certain serum lipoproteins in a manner similar to in vitro complexing of lipoproteins and GAG in the presence of Ca2+.
从人主动脉的纤维斑块病变中分离出脂蛋白 - 糖胺聚糖（GAG）复合物，并通过电子显微镜进行检查。经凝胶色谱和超速离心分级分离后，极低密度脂蛋白 - GAG和低密度脂蛋白 - GAG复合物均显示出主要直径为1000至2000埃的颗粒。在凝胶过滤后以及复合物的极低密度脂蛋白组分中偶尔可见直径为3000至10000埃的大聚集体。一般来说，这些复合物比血清极低密度脂蛋白和低密度脂蛋白大，尽管血清极低密度脂蛋白颗粒直径范围为250至2000埃。体外低密度脂蛋白 - 肝素复合物由通常直径为1000至2000埃的球形颗粒组成，这些颗粒是在肝素和Ca2 +存在下较小的低密度脂蛋白颗粒聚集和合并形成的。这些观察结果支持这样一种概念，即主动脉内膜“基质”中的GAG以类似于体外脂蛋白与GAG在Ca2 +存在下复合的方式隔离某些血清脂蛋白。
