Li Xin, Hu Lan, Feng Xue-fei, Liu Xiao
Chinese Peoples Public Security University, Beijing 100038, China.
Fa Yi Xue Za Zhi. 2007 Aug;23(4):286-9.
To establish a method of preparing and analyzing DNA of sperm cells from sample mixtures.
Sperm cells were captured by micromanipulation method, DNA template was prepared from these sperm cells, and then amplified by multiple displacement amplification (MDA).
High-yield of highly integrated DNA was produced by MDA from very small amount of DNA template of 20 sperm cells. The DNA could be amplified at least 30,000-fold, and the length of most the DNA segments were greater than 15 kb, providing sufficient amount of short tandem repeat (STR) products for DNA genotyping.
The micromanipulation method is efficient to isolate sperm cells from sample mixtures and multiple displacement amplification can provide sufficient amount of DNA products for STR genotyping. The method seems to be feasible in detecting DNA from rape cases.
建立一种从混合样本中制备和分析精子细胞DNA的方法。
采用显微操作法捕获精子细胞，以这些精子细胞制备DNA模板，然后通过多重置换扩增（MDA）进行扩增。
通过MDA从20个精子细胞的极少量DNA模板中产生了高产率的高度完整的DNA。该DNA可扩增至少30000倍，且大多数DNA片段长度大于15kb，为DNA基因分型提供了足够量的短串联重复序列（STR）产物。
显微操作法能有效地从混合样本中分离精子细胞，多重置换扩增可为STR基因分型提供足够量的DNA产物。该方法在检测强奸案件的DNA方面似乎是可行的。
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