Bauer A, Rørvik L M
Norwegian School of Veterinary Science, Department of Food Safety and Infection Biology, Oslo, Norway.
Lett Appl Microbiol. 2007 Oct;45(4):371-5. doi: 10.1111/j.1472-765X.2007.02195.x.
To establish a simple multiplex polymerase chain reaction (PCR) that will identify Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
A total of 429 Vibrio spp. from various origins were tested with the novel primers targeting toxR. The reverse primers were all designed to be species specific, while the forward primer was universal. The primers correctly identified all the V. parahaemolyticus, V. cholerae and V. vulnificus isolates tested.
The toxR multiplex PCR works well when the initial colony morphology is known. If not, Vibrio alginolyticus might represent a diagnostic obstacle.
The method provides a fast and reliable way of identifying the main Vibrio spp. involved in food-borne disease. The method could prove very useful for laboratories working with identification of these Vibrio spp.
建立一种简单的多重聚合酶链反应（PCR），用于鉴定副溶血性弧菌、霍乱弧菌和创伤弧菌。
用靶向toxR的新型引物对总共429株来自不同来源的弧菌属菌株进行检测。反向引物均设计为种特异性引物，而正向引物为通用引物。这些引物正确鉴定了所有检测的副溶血性弧菌、霍乱弧菌和创伤弧菌分离株。
当已知初始菌落形态时，toxR多重PCR效果良好。如果未知，溶藻弧菌可能会成为诊断障碍。
该方法提供了一种快速可靠的方法来鉴定食源性疾病中涉及的主要弧菌属菌种。该方法对从事这些弧菌属菌种鉴定的实验室可能非常有用。
